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| Screening and Characterization of a Salt-tolerant PAHs-degrading Strain Isolated from Gasoline Contaminated Soil[J]. Research Journal of BioTechnology. 2015, 10(7): 40-44 |
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https://apps.webofknowledge.com/ ... age=1&doc=1 Screening and Characterization of a Salt-tolerant PAHs-degrading Strain isolated from Gasoline contaminated Soil ×÷Õß:Zhang, XS (Zhang Xiangsheng)[ 1,2 ] RESEARCH JOURNAL OF BIOTECHNOLOGY ¾í: 10 ÆÚ: 8 Ò³: 82-86 ³ö°æÄê: AUG 2015 ²é¿´ÆÚ¿¯ÐÅÏ¢ RESEARCH JOURNAL OF BIOTECHNOLOGY ³ö°æÉÌ RESEARCH JOURNAL BIOTECHNOLOGY, SECTOR A-80, SCHEME NO 54, VIJAY NAGAR, A B ROAD, INDORE, 452 010 MP, INDIA ISSN: 2278-4535 Ñо¿ÁìÓò Biotechnology & Applied Microbiology ÕªÒª In this study, three PAHs (anthracene, phenanthrene and pyrene) were employed as substrates to isolate PAHs degrading microbial strains. A bacterial strain MN1 was screened out from soil sample nearby a bus gas station using plate screening techniques with PAHs as sole carbon and energy source. The bacterial strain could grow well on the screening agar plate with 800mg/L PAHs and 3.5% NaCl. With physiological and biochemical analyses and 16S rRNA gene sequence analysis, MN1 was identified as Pseudomonas stutzeri. MN1 performed well in degrading PAHs in aerobic shaking flasks. After 144h fermentation in shaking flasks, the biotic degradation rate of PAHs at 3.5% salinity by MN1 was 41.1% (anthracene and phenanthrene) and 44.6% (pyrene) respectively in 50mg/L-degrading culture media, with better degrading capacity of pyrene than that of anthracene and phenanthrene. This study gives hints for further study in PAHs aerobic biodegradation under saline conditions. ¹Ø¼ü´Ê ×÷Õ߹ؼü´Ê seudomonas stutzeri; Anthracene; PAHs; Phenanthrene; Pyrene; Screening; Aerobic degradation KeyWords Plus OLYCYCLIC AROMATIC-HYDROCARBONS; DEGRADATION; BIODEGRADATION; FLUORANTHENE; PHENANTHRENE; FLUORENE; PYRENE ×÷ÕßÐÅÏ¢ ͨѶ×÷ÕßµØÖ·: Zhang, XS (ͨѶ×÷Õß) Yancheng Teachers Univ, Jiangsu Prov Key Lab Coastal Wetland Bioresources, Yancheng 224051, Peoples R China. µØÖ·: [ 1 ] Yancheng Teachers Univ, Jiangsu Prov Key Lab Coastal Wetland Bioresources, Yancheng 224051, Peoples R China [ 2 ] Yancheng Teachers Univ, Jiangsu Key Lab Bioresources Saline Soils, Yancheng 224051, Peoples R China µç×ÓÓʼþµØÖ·:yongshengzh@163.com »ù½ð×ÊÖúÖÂл »ù½ð×ÊÖú»ú¹¹ ÊÚȨºÅ Open Project of Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection Jiangsu Key Laboratory for Bioresources of Saline Soil Six Talents Peak Project of Jiangsu Province SWYY-018 ²é¿´»ù½ð×ÊÖúÐÅÏ¢¹Ø±Õ»ù½ð×ÊÖúÐÅÏ¢ This research was supported by Open Project of Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection and Jiangsu Key Laboratory for Bioresources of Saline Soil and Six Talents Peak Project of Jiangsu Province (SWYY-018) ³ö°æÉÌ RESEARCH JOURNAL BIOTECHNOLOGY, SECTOR A-80, SCHEME NO 54, VIJAY NAGAR, A B ROAD, INDORE, 452 010 MP, INDIA Àà±ð / ·ÖÀà Ñо¿·½Ïò:Biotechnology & Applied Microbiology Web of Science Àà±ð:Biotechnology & Applied Microbiology ÎÄÏ×ÐÅÏ¢ ÎÄÏ×ÀàÐÍ:Article ÓïÖÖ:English Èë²ØºÅ: WOS:000358572700011 ISSN: 2278-4535 ÆäËûÐÅÏ¢ IDS ºÅ: CN6VO Web of Science ºËÐĺϼ¯ÖÐµÄ "ÒýÓõIJο¼ÎÄÏ×": 15 Web of Science ºËÐĺϼ¯ÖÐµÄ "±»ÒýƵ´Î": 0 JCR® Àà±ð JCR ·ÖÇø BIOTECHNOLOGY & APPLIED MICROBIOLOGY Q4 Êý¾ÝÀ´×ÔµÚ 2014 °æ Journal Citation Reports® |
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seudomonas stutzeri; Anthracene; PAHs; Phenanthrene; Pyrene; Screening; Aerobic degradation
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