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【答案】应助回帖
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Screening and Characterization of a Salt-tolerant PAHs-degrading Strain isolated from Gasoline contaminated Soil
作者:Zhang, XS (Zhang Xiangsheng)[ 1,2 ]
RESEARCH JOURNAL OF BIOTECHNOLOGY
卷: 10
期: 8
页: 82-86
出版年: AUG 2015
查看期刊信息
RESEARCH JOURNAL OF BIOTECHNOLOGY
出版商 RESEARCH JOURNAL BIOTECHNOLOGY, SECTOR A-80, SCHEME NO 54, VIJAY NAGAR, A B ROAD, INDORE, 452 010 MP, INDIA
ISSN: 2278-4535
研究领域 Biotechnology & Applied Microbiology
摘要
In this study, three PAHs (anthracene, phenanthrene and pyrene) were employed as substrates to isolate PAHs degrading microbial strains. A bacterial strain MN1 was screened out from soil sample nearby a bus gas station using plate screening techniques with PAHs as sole carbon and energy source. The bacterial strain could grow well on the screening agar plate with 800mg/L PAHs and 3.5% NaCl. With physiological and biochemical analyses and 16S rRNA gene sequence analysis, MN1 was identified as Pseudomonas stutzeri. MN1 performed well in degrading PAHs in aerobic shaking flasks.
After 144h fermentation in shaking flasks, the biotic degradation rate of PAHs at 3.5% salinity by MN1 was 41.1% (anthracene and phenanthrene) and 44.6% (pyrene) respectively in 50mg/L-degrading culture media, with better degrading capacity of pyrene than that of anthracene and phenanthrene. This study gives hints for further study in PAHs aerobic biodegradation under saline conditions.
关键词
作者关键词 seudomonas stutzeri; Anthracene; PAHs; Phenanthrene; Pyrene; Screening; Aerobic degradation
KeyWords Plus OLYCYCLIC AROMATIC-HYDROCARBONS; DEGRADATION; BIODEGRADATION; FLUORANTHENE; PHENANTHRENE; FLUORENE; PYRENE
作者信息
通讯作者地址: Zhang, XS (通讯作者) Yancheng Teachers Univ, Jiangsu Prov Key Lab Coastal Wetland Bioresources, Yancheng 224051, Peoples R China.
地址: [ 1 ] Yancheng Teachers Univ, Jiangsu Prov Key Lab Coastal Wetland Bioresources, Yancheng 224051, Peoples R China
[ 2 ] Yancheng Teachers Univ, Jiangsu Key Lab Bioresources Saline Soils, Yancheng 224051, Peoples R China
电子邮件地址:yongshengzh@163.com
基金资助致谢
基金资助机构 授权号
Open Project of Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection
Jiangsu Key Laboratory for Bioresources of Saline Soil
Six Talents Peak Project of Jiangsu Province SWYY-018
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This research was supported by Open Project of Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection and Jiangsu Key Laboratory for Bioresources of Saline Soil and Six Talents Peak Project of Jiangsu Province (SWYY-018)
出版商
RESEARCH JOURNAL BIOTECHNOLOGY, SECTOR A-80, SCHEME NO 54, VIJAY NAGAR, A B ROAD, INDORE, 452 010 MP, INDIA
类别 / 分类
研究方向:Biotechnology & Applied Microbiology
Web of Science 类别:Biotechnology & Applied Microbiology
文献信息
文献类型:Article
语种:English
入藏号: WOS:000358572700011
ISSN: 2278-4535
其他信息
IDS 号: CN6VO
Web of Science 核心合集中的 "引用的参考文献": 15
Web of Science 核心合集中的 "被引频次": 0
JCR® 类别 JCR 分区
BIOTECHNOLOGY & APPLIED MICROBIOLOGY Q4
数据来自第 2014 版 Journal Citation Reports® |
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