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EIÊÕ¼ Accession number: 20152801022864 Title: Oxidative stress damage from nanometer titanium dioxide in goldfish brain tissue Authors: Sun, Huiqun1 Email author sunhqliuzh@163.com; Zhang, Qun1; Chen, Shuqin1; Cao, Jingjing1 Author affiliation: 1 Resources and Environment Department of Anqing Teachers College, Anqing, China Source title: Research of Environmental Sciences Abbreviated source title: Res. Environ. Sci. Volume: 28 Issue: 6 Issue date: June 1, 2015 Publication year: 2015 Pages: 930-935 Language: Chinese ISSN: 10016929 Document type: Journal article (JA) Publisher: Editorial department of Molecular Catalysis, Tianshui Road No. 18, Lanzhou City, Gansu Province,, 730000, China Abstract: In order to investigate the oxidative damage mechanism of photocatalytic type nano-TiO<inf>2</inf> on goldfish brain tissue, a test involving the content of malondialdehyde (MDA), protein carbonyl (PC) and H<inf>2</inf>O<inf>2</inf> in goldfish brain tissues together with the activity of superoxide dismutase (SOD) and glutathione peroxidase (GPx) and the expression of Cu/Zn-SOD protein was determined after goldfishes were exposed to different nano-TiO<inf>2</inf> concentrations of 0, 16, 32, 64 and 128 mg/L. The results showed that the Cu/Zn-SOD activity in goldfish brain tissues decreased from 1.70¡Á10<sup>-3</sup> kat/kg to 0.35¡Á10<sup>-3</sup> kat/kg, and then rebounded to 0.46¡Á10<sup>-3</sup> kat/kg with the nano-TiO<inf>2</inf> concentration varying from 0 to 128 mg/L. Meanwhile, the Mn-SOD activity presented a decrease-increase-decrease trend; the MDA content increased from 0.85 to 2.83 nmol/g, and then decreased to 2.54 nmol/g. The PC content increased all the way when the H<inf>2</inf>O<inf>2</inf> content increased from 4.32 to 9.28 ¦Ìmol/g, and then decreased to 8.38 ¦Ìmol/g. When the nano-TiO<inf>2</inf> concentration increased, the expression of Cu/Zn-SOD decreased gradually. The research indicated that nano-TiO<inf>2</inf> strongly affected the oxidative damage in goldfish brain tissues. The increase of the MDA and PC content was associated with the decreasing of the Cu/Zn-SOD and GPx activity, while H<inf>2</inf>O<inf>2</inf> has a bearing on the GPx activity and other factors. ©, 2015, Editorial department of Molecular Catalysis. All right reserved. Number of references: 32 Main heading: Brain Controlled terms: Aldehydes - Enzymes - Gold - Histology - Manganese - Oxides - Oxygen - Peptides - Plants (botany) - Proteins - Tissue - Titanium - Titanium dioxide Uncontrolled terms: Glutathione peroxidase - Malondialdehyde - Nanometer titanium dioxide - Oxidative damage - Protein carbonyls - Super oxide dismutase Classification code: 461 Bioengineering and Biology - 542.3 Titanium and Alloys - 543.2 Manganese and Alloys - 547.1 Precious Metals - 804 Chemical Products Generally DOI: 10.13198/j.issn.1001-6929.2015.06.13 Database: Compendex Compilation and indexing terms, © 2015 Elsevier Inc. |

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