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| Several bacteriological media are available for selectively detecting gram-negative bacteria in dairy products. Crystal violet tetrazolium agar (CVTA; Frank and Yousef, 2004), MacConkey agar (MAC; Difco, 1998), violet red bile agar (VRBA; Frank and Yousef, 2004), and 3M Coliform Petrifilm (CPETRI, 3M, St. Paul, MN) are examples of gram-negative selective media. Crystal violet tetrazolium agar, MAC, and VRBA contain crystal violet to inhibit the growth of grampositive bacteria, and MAC and VRBA also contain bile salts, which may inhibit some gram-negative organisms; therefore, CVTA is the recommended method for the detection of total gram-negative bacteria in milk (Frank and Yousef, 2004). Violet red bile agar and MAC also contain lactose and a pH indicator and can thus be used to differentiate non-lactose-fermenters from lactose fermenters, which include coliform bacteria. Coliform Petrifilm is designed to detect specifically coliform bacteria and is widely used in the dairy industry. |
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