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【答案】应助回帖
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中国的_BOY: 金币+50, 翻译EPI+1, ★★★★★最佳答案, 很好的 2015-10-21 15:57:56
Several bacteriological media are available for selectively detecting gram-negative bacteria in dairy products. Crystal violet tetrazolium agar (CVTA; Frank and Yousef, 2004), MacConkey agar (MAC; Difco, 1998), violet red bile agar (VRBA; Frank and Yousef, 2004), and 3M Coliform Petrifilm (CPETRI, 3M, St. Paul, MN) are examples of gram-negative selective media. Crystal violet tetrazolium agar, MAC, and VRBA contain crystal violet to inhibit the growth of grampositive bacteria, and MAC and VRBA also contain bile salts, which may inhibit some gram-negative organisms; therefore, CVTA is the recommended method for the detection of total gram-negative bacteria in milk (Frank and Yousef, 2004). Violet red bile agar and MAC also contain lactose and a pH indicator and can thus be used to differentiate non-lactose-fermenters from lactose fermenters, which include coliform bacteria. Coliform Petrifilm is designed to detect specifically coliform bacteria and is widely used in the dairy industry.
有多个细菌培养基可用于检测乳制品中的革兰氏阴性细菌，结晶紫四唑琼脂(CVTA;Frank and Yousef,2004)，麦康凯琼脂(MAC;Difco,1998)，结晶紫中性红胆盐琼脂(VRBA;Frank and Yousef,2004)和3M大肠菌测试片(CPETRI、3M、St. Paul, MN)等都可以。结晶紫四唑琼脂,MAC和VRBA含有抑制革兰氏阳性菌生长的结晶紫，另外，MAC和VRBA均含有胆盐，而胆盐可以抑制某些革兰氏阴性细菌，因此，检测乳制品总革兰氏阴性细菌时推荐采用CVTA法(Frank and Yousef,2004)。结晶紫中性红胆盐琼脂和MAC均含有乳糖和酸碱指示剂，因此可用于区分非乳糖发酵菌和乳糖发酵菌，后者包括大肠菌。大肠菌测试片专用于检测大肠菌，在乳制品行业中广泛应用。 |
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