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lastmonday

捐助贵宾 (初入文坛)

[求助] 化学方面的英译汉,求专业,准确。

Gels were formed by mixing the chosen PEG-acrylate with
either dithiothreitol (DTT, Sigma) or PEG-dithiol, Mw ) 3.1
kD (Shearwater Polymers) at a 1:1 stoichiometric ratio of
acrylates to thiols in 50 mM phosphate buffered solution
(PBS, pH 7.8). Briefly, appropriate amounts of each reactant
were dissolved separately in an aliquot of PBS. The amount
of PBS was varied to give the desired total precursor
concentration (wt %) upon mixing. The two solutions were
mixed vigorously in 1.5 mL plastic tubes and centrifuged
briefly to remove bubbles. The sealed tube containing the
mixture was then placed at 37 °C, and the gel was allowed
to react overnight to ensure complete conversion. For almost
all systems, gelation was observed visually within a few
minutes of mixing.

特别是其中的50mM,vigorously应该怎么理解?
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ssssllllnnnn

至尊木虫 (知名作家)

Translator and Proofreader


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lastmonday: 金币+15, 翻译EPI+1, ★★★很有帮助, 谢谢 2015-10-19 14:29:25
我要睡了,不能给你翻了。
50 mM是50 mmol/L,即摩尔浓度。具体到你这里是指PBS中磷酸盐的浓度为50 mM。
vigorously是指混匀的时候的程度,即剧烈地。这里显然是指在1.5 ml Eppendorf管中非常剧烈的混匀(通常是vortexing这个动作)
2楼2015-10-19 14:13:49
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sdhuai

金虫 (著名写手)

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50mM,是50毫摩尔的意思;
vigorously 是剧烈摇晃的意思
3楼2015-10-20 13:44:47
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