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hua20087125: 金币+20, 翻译EPI+1, ★★★★★最佳答案, 非常好，感谢！ 2015-08-30 18:10:41
DNA isolation is a very error prone step.Significant different results might be obtained with different extraction methods and reagents. In addition, different researchers can also get dramatic different results with the same method and samples. It has been reported by many researchers that the recovery rate of DNA with different methods is quite different, in some cases, the difference is even up to several times. Moreover, most of the small DNA fragments will be lost when the DNA is purified with some commercial DNA isolation kits.
大致按你的意思写的。
也许你这是在回答审稿人的意见。我不知道具体意见是什么，但是，如果我是审稿人，对这样的回答是不能满意的。DNA的分离纯化是个非常成熟的过程，你所说的每一点都有其可能性，但就这个方法来说并不会造成什么后果。对于DNA纯化而言，产量、回收率都不是什么问题，相对重要的是降解问题，即便后者，其重要性也要看纯化的目的。所以，你要看看审稿人的具体意见，没有必要把问题扩大化、严重化。 |
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