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岁月微醺

金虫 (小有名气)

[交流] 请问有做细胞浆/核蛋白提取的朋友吗?求指导分析

最近在做这个,都是按照说明书做的,可是蛋白含量一直很低,而且曝光检测不到目的条带,请问是怎么回事啊?该如何改正?
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岁月微醺

金虫 (小有名气)

引用回帖:
6楼: Originally posted by fuyuandj86 at 2015-08-27 09:06:32
是核蛋白浓度低吗?核蛋白浓度低很正常。一般分离以后细胞质蛋白是细胞核蛋白总量的5-10倍。
你用的核蛋白的内参是什么?核蛋白不能用GAPDH做内参的

我用的是lamin B
8楼2015-08-27 12:48:08
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hc-material

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小木虫: 金币+0.5, 给个红包,谢谢回帖
给的信息太少,不好分析。
2楼2015-08-25 17:15:23
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gJordys

新虫 (初入文坛)

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小木虫: 金币+0.5, 给个红包,谢谢回帖
岁月微醺: 金币+5 2015-08-27 12:46:14
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3楼2015-08-25 19:42:29
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gJordys

新虫 (初入文坛)

Synergetic effect of Andrographis paniculata polysaccharide on diabetic nephropathy with andrographolide
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Jie Xu, Zhanting Li, Min Cao, Han Zhang, Jifeng Sun, Jie Zhao, Qiang Zhou, Zhifen Wu, Lianjia Yang
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International Journal of Biological Macromolecules, Volume 52, January 2013, Pages 280-285
Guang Zeng, Ying Ju, Huan Shen, Ningxin Zhou, Li Huang
Auricularia polytricha polysaccharides induce cell cycle arrest and apoptosis in human lung cancer A549 cells
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Jie Yu, Ruilin Sun, Zhongquan Zhao, Yingyu Wang
4楼2015-08-25 19:43:32
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