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岁月微醺

金虫 (小有名气)

[交流] 请问有做细胞浆/核蛋白提取的朋友吗?求指导分析

最近在做这个,都是按照说明书做的,可是蛋白含量一直很低,而且曝光检测不到目的条带,请问是怎么回事啊?该如何改正?
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fuyuandj86

版主 (著名写手)

己所不欲,勿施于人


小木虫: 金币+0.5, 给个红包,谢谢回帖
是核蛋白浓度低吗?核蛋白浓度低很正常。一般分离以后细胞质蛋白是细胞核蛋白总量的5-10倍。
你用的核蛋白的内参是什么?核蛋白不能用GAPDH做内参的
The doer of good becomes good, the doer of evil becomes evil.
6楼2015-08-27 09:06:32
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hc-material

专家顾问 (职业作家)


小木虫: 金币+0.5, 给个红包,谢谢回帖
给的信息太少,不好分析。
2楼2015-08-25 17:15:23
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gJordys

新虫 (初入文坛)

★ ★ ★ ★ ★ ★
小木虫: 金币+0.5, 给个红包,谢谢回帖
岁月微醺: 金币+5 2015-08-27 12:46:14
Antitumor and immunomodulating activity of a polysaccharide from Sophora flavescens Ait.
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Lu Bai, Li-Ying Zhu, Bao-Shan Yang, Li-Jun Shi, Yong Liu, Ai-Min Jiang, Li-Li Zhao, Guang Song, Tie-Fu Liu
Immunological response in H22 transplanted mice undergoing Aconitum coreanum polysaccharide treatment
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Purification, characterization and anticancer activity of a polysaccharide from Panax ginseng
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3楼2015-08-25 19:42:29
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gJordys

新虫 (初入文坛)

Synergetic effect of Andrographis paniculata polysaccharide on diabetic nephropathy with andrographolide
Original Research Article
International Journal of Biological Macromolecules, Volume 51, Issue 5, December 2012, Pages 738-742
Jie Xu, Zhanting Li, Min Cao, Han Zhang, Jifeng Sun, Jie Zhao, Qiang Zhou, Zhifen Wu, Lianjia Yang
Anti-hepatoma activity and mechanism of corn silk polysaccharides in H22 tumor-bearing mice
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International Journal of Biological Macromolecules, Volume 64, March 2014, Pages 276-280
Jingyue Yang, Xiao Li, Yan Xue, Nan Wang, Wenchao Liu
A polysaccharide from Sanguisorbae radix induces caspase-dependent apoptosis in human leukemia HL-60 cells
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International Journal of Biological Macromolecules, Volume 70, September 2014, Pages 615-620
Zhigang Wu, Honghui Sun, Jingzhong Li, Chijiao Ma, Siqiao Zhao, Zheng Guo, Yao Lin, Yaping Lin, Li Liu
Immunopontentiating activities of the purified polysaccharide from evening primrose in H22 tumor-bearing mice
Original Research Article
International Journal of Biological Macromolecules, Volume 52, January 2013, Pages 280-285
Guang Zeng, Ying Ju, Huan Shen, Ningxin Zhou, Li Huang
Auricularia polytricha polysaccharides induce cell cycle arrest and apoptosis in human lung cancer A549 cells
Original Research Article
International Journal of Biological Macromolecules, Volume 68, July 2014, Pages 67-71
Jie Yu, Ruilin Sun, Zhongquan Zhao, Yingyu Wang
4楼2015-08-25 19:43:32
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