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岁月微醺金虫 (小有名气)
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[交流]
请问有做细胞浆/核蛋白提取的朋友吗？求指导分析
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| 最近在做这个，都是按照说明书做的，可是蛋白含量一直很低，而且曝光检测不到目的条带，请问是怎么回事啊？该如何改正？ |
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Synergetic effect of Andrographis paniculata polysaccharide on diabetic nephropathy with andrographolide Original Research Article International Journal of Biological Macromolecules, Volume 51, Issue 5, December 2012, Pages 738-742 Jie Xu, Zhanting Li, Min Cao, Han Zhang, Jifeng Sun, Jie Zhao, Qiang Zhou, Zhifen Wu, Lianjia Yang Anti-hepatoma activity and mechanism of corn silk polysaccharides in H22 tumor-bearing mice Original Research Article International Journal of Biological Macromolecules, Volume 64, March 2014, Pages 276-280 Jingyue Yang, Xiao Li, Yan Xue, Nan Wang, Wenchao Liu A polysaccharide from Sanguisorbae radix induces caspase-dependent apoptosis in human leukemia HL-60 cells Original Research Article International Journal of Biological Macromolecules, Volume 70, September 2014, Pages 615-620 Zhigang Wu, Honghui Sun, Jingzhong Li, Chijiao Ma, Siqiao Zhao, Zheng Guo, Yao Lin, Yaping Lin, Li Liu Immunopontentiating activities of the purified polysaccharide from evening primrose in H22 tumor-bearing mice Original Research Article International Journal of Biological Macromolecules, Volume 52, January 2013, Pages 280-285 Guang Zeng, Ying Ju, Huan Shen, Ningxin Zhou, Li Huang Auricularia polytricha polysaccharides induce cell cycle arrest and apoptosis in human lung cancer A549 cells Original Research Article International Journal of Biological Macromolecules, Volume 68, July 2014, Pages 67-71 Jie Yu, Ruilin Sun, Zhongquan Zhao, Yingyu Wang |
4楼2015-08-25 19:43:32
hc-material
专家顾问 (职业作家)
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专家经验: +702 - BioEPI: 1
- 应助: 517 (博士)
- 金币: 467.7
- 散金: 18028
- 红花: 313
- 帖子: 3594
- 在线: 518.8小时
- 虫号: 4027804
- 注册: 2015-08-18
- 性别: GG
- 专业: 核技术及其应用
- 管辖: 生物科学
2楼2015-08-25 17:15:23
★ ★ ★ ★ ★ ★
小木虫: 金币+0.5, 给个红包，谢谢回帖
岁月微醺: 金币+5 2015-08-27 12:46:14
小木虫: 金币+0.5, 给个红包，谢谢回帖
岁月微醺: 金币+5 2015-08-27 12:46:14
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Antitumor and immunomodulating activity of a polysaccharide from Sophora flavescens Ait. Original Research Article International Journal of Biological Macromolecules, Volume 51, Issue 5, December 2012, Pages 705-709 Lu Bai, Li-Ying Zhu, Bao-Shan Yang, Li-Jun Shi, Yong Liu, Ai-Min Jiang, Li-Li Zhao, Guang Song, Tie-Fu Liu Immunological response in H22 transplanted mice undergoing Aconitum coreanum polysaccharide treatment Original Research Article International Journal of Biological Macromolecules, Volume 55, April 2013, Pages 295-300 Huisheng Li, Meina Sun, Jin Xu, Hui Li, Meng Zang, Yunfu Cui Antitumor activity of polysaccharides from Lepista sordida against laryngocarcinoma in vitro and in vivo Original Research Article International Journal of Biological Macromolecules, Volume 60, September 2013, Pages 235-240 Susheng Miao, Xionghui Mao, Rong Pei, Suping Miao, Cheng Xiang, Yuanjing Lv, Xianguang Yang, Ji Sun, Shenshan Jia, Yvpeng Liu Antitumor and antiangiogenic activity of Schisandra chinensis polysaccharide in a renal cell carcinoma model Original Research Article International Journal of Biological Macromolecules, Volume 66, May 2014, Pages 52-56 Hai-Ming Qu, Shi-Jian Liu, Chun-Ying Zhang Immunopontentiating and antitumor activities of a polysaccharide from Pulsatilla chinensis (Bunge) Regel Original Research Article International Journal of Biological Macromolecules, Volume 54, March 2013, Pages 225-229 Tong Liu, Leiguang Ye, Xueqing Guan, Xiaoshuan Liang, Cong Li, Qian Sun, Yang Liu, Shiru Chen, Fa Bang, Baogang Liu Panax ginseng polysaccharide suppresses metastasis via modulating Twist expression in gastric cancer Original Research Article International Journal of Biological Macromolecules, Volume 57, June 2013, Pages 22-25 Jian-Ping Cai, Yang-Jiazi Wu, Cong Li, Mei-Yan Feng, Qing-Tao Shi, Rui Li, Zhen-Yu Wang, Jing-Shu Geng Purification, characterization and anticancer activity of a polysaccharide from Panax ginseng Original Research Article International Journal of Biological Macromolecules, Volume 51, Issue 5, December 2012, Pages 968-973 Cong Li, Jianping Cai, Jingshu Geng, Yinghong Li, Zhenyu Wang, Rui Li |
3楼2015-08-25 19:42:29
★
小木虫: 金币+0.5, 给个红包，谢谢回帖
小木虫: 金币+0.5, 给个红包，谢谢回帖
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Antioxidant activities of different polysaccharide conjugates (CRPs) isolated from the fruiting bodies of Chroogomphis rutilus (Schaeff.: Fr.) O. K. MillerOriginal Research Article Carbohydrate Polymers, Volume 82, Issue 2, 5 September 2010, Pages 510-514 Yong-Xu Sun, John F. Kennedy Purification, structural analysis and hydroxyl radical-scavenging capacity of a polysaccharide from the fruiting bodies of Russula virescensOriginal Research Article Process Biochemistry, Volume 45, Issue 6, June 2010, Pages 874-879 Yong-Xu Sun, Ji-Cheng Liu, Xiu-Dong Yang, John F. Kennedy |
5楼2015-08-25 19:45:32