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1 BioBrick Assembly Standards and Techniques ...
2  Sequence and Ligation-  Independent Cloning ......
3 Quick and Clean Cloning .............
4 Hierarchical Ligation-Independent Assembly of PCR Fragments ..........
6 Restriction-Free (RF) Cloning for Multicomponents Assembly ............... 73
7 A Single-Tube Assembly of DNA Using the Transfer-PCR (TPCR) Platform ................... 89
8 Circular Polymerase Extension Cloning ............................................................ 103
9 Golden Gate Cloning ....................................................................................... 119
10  GoldenBraid Cloning Strategy .................... 133
11 FX Cloning... 153
12 Minimum GC-Rich Sequences for Overlap Extension PCR and Primer Annealing ............ 165
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  • 2015-08-09 02:43:33, 9.01 M

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