²é¿´: 3636  |  »Ø¸´: 76
¡¾½±Àø¡¿ ±¾Ìû±»ÆÀ¼Û73´Î£¬×÷ÕßliygmailÔö¼Ó½ð±Ò 57.8 ¸ö

liygmail

ÖÁ×ðľ³æ (Ö°Òµ×÷¼Ò)


[×ÊÔ´] 2014ÄêÔ­°æµç×ÓÊé------DNA¿Ë¡ºÍ×é×°

2014ÄêÔ­°æµç×ÓÊé------DNA¿Ë¡ºÍ×é×°
ÄÚÈÝ£º
1 BioBrick Assembly Standards and Techniques ...
2  Sequence and Ligation-  Independent Cloning ......
3 Quick and Clean Cloning .............
4 Hierarchical Ligation-Independent Assembly of PCR Fragments ..........
6 Restriction-Free (RF) Cloning for Multicomponents Assembly ............... 73
7 A Single-Tube Assembly of DNA Using the Transfer-PCR (TPCR) Platform ................... 89
8 Circular Polymerase Extension Cloning ............................................................ 103
9 Golden Gate Cloning ....................................................................................... 119
10  GoldenBraid Cloning Strategy .................... 133
11 FX Cloning... 153
12 Minimum GC-Rich Sequences for Overlap Extension PCR and Primer Annealing ............ 165
...................
±¾·ÖÏí½öÓÃÓÚ½»Á÷ѧϰ£¬Èç¹û×ÊÔ´ÓÐÓã¬ÇëÓèÖÃÆÀ2014ÄêÔ­°æµç×ÓÊé------DNA¿Ë¡ºÍ×é×°
2015-08-08_124341.jpg
»Ø¸´´ËÂ¥

» ±¾Ìû¸½¼þ×ÊÔ´Áбí

  • »¶Ó­¼à¶½ºÍ·´À¡£ºÐ¡Ä¾³æ½öÌṩ½»Á÷ƽ̨£¬²»¶Ô¸ÃÄÚÈݸºÔð¡£
    ±¾ÄÚÈÝÓÉÓû§×ÔÖ÷·¢²¼£¬Èç¹ûÆäÄÚÈÝÉæ¼°µ½ÖªÊ¶²úȨÎÊÌ⣬ÆäÔðÈÎÔÚÓÚÓû§±¾ÈË£¬Èç¶Ô°æÈ¨ÓÐÒìÒ飬ÇëÁªÏµÓÊÏ䣺xiaomuchong@tal.com
  • ¸½¼þ 1 : DNA_Cloning_and_Assembly_Methods-2014.pdf
  • 2015-08-09 02:43:33, 9.01 M

» ÊÕ¼±¾ÌûµÄÌÔÌûר¼­ÍƼö

Êé¼®ÏÂÔØÍøÕ¾ רҵÊé¼®£¨ÍâÎİ棩WM ÉúÎïÒ½Ò©ÎÄÏ×Êé ÉúÎïҽѧ×ÊÁÏ
Biotech×ÊÁÏ ÄãÖµµÃÓµÓÐ ºÏ³ÉÉúÎïѧ

» ²ÂÄãϲ»¶

» ±¾Ö÷ÌâÏà¹Ø¼ÛÖµÌùÍÆ¼ö£¬¶ÔÄúͬÑùÓаïÖú:

ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

ÎÕ²»ÎÔ

гæ (³õÈëÎÄ̳)


¡ï¡ï¡ï¡ï¡ï ÎåÐǼ¶,ÓÅÐãÍÆ¼ö

顶一下,感谢分享�

[ ·¢×ÔСľ³æ¿Í»§¶Ë ]
8Â¥2015-08-10 01:24:58
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

zhangh62

ľ³æ (ÖøÃûдÊÖ)


¡ï¡ï¡ï¡ï¡ï ÎåÐǼ¶,ÓÅÐãÍÆ¼ö

ºÃÊ飬̫¸Ðл¥Ö÷À²£¡
9Â¥2015-08-10 11:08:56
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
¼òµ¥»Ø¸´
2015-08-09 07:26   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
jinhx873Â¥
2015-08-09 09:12   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
wzhsteve4Â¥
2015-08-09 13:19   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
xujian5685Â¥
2015-08-09 13:31   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
2015-08-09 17:27   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
1592364787Â¥
2015-08-09 17:57   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
lingshao40510Â¥
2015-08-10 11:22   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
VictoryDai11Â¥
2015-08-10 11:34   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
Goodman299512Â¥
2015-08-10 13:25   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
¶À¹Â²Ï13Â¥
2015-08-10 17:50   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
jianghy6914Â¥
2015-08-10 18:02   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
meilleur3315Â¥
2015-08-10 18:27   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
haozy8716Â¥
2015-08-10 23:01   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
shengdeqiao17Â¥
2015-08-11 09:24   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
daoke200518Â¥
2015-08-11 10:00   »Ø¸´  
ÈýÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
2015-08-11 10:31   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
maciter20Â¥
2015-08-11 10:58   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
lieying1821Â¥
2015-08-11 11:12   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
2015-08-11 11:13   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
zhchzhsh207623Â¥
2015-08-11 13:12   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
luntanhu24Â¥
2015-08-11 13:17   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
gogo25Â¥
2015-08-11 15:03   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
weishuang9826Â¥
2015-08-11 15:14   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
ha166827Â¥
2015-08-11 16:12   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
Q½À02128Â¥
2015-08-11 16:27   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
wildfire203829Â¥
2015-08-11 17:32   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
zhouxj30Â¥
2015-08-11 17:42   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
limingmaster31Â¥
2015-08-11 19:03   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
wangmingsh32Â¥
2015-08-11 21:22   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
biosensors33Â¥
2015-08-12 15:24   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
cellman34Â¥
2015-08-12 16:05   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
wmnick35Â¥
2015-08-12 21:25   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
2015-08-13 08:34   »Ø¸´  
¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
yuanchang37Â¥
2015-08-13 09:12   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
daiminzh38Â¥
2015-08-13 09:23   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
chieflee39Â¥
2015-08-13 14:40   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
lindoudou40Â¥
2015-08-13 17:19   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
everie41Â¥
2015-08-14 01:04   »Ø¸´  
ÎåÐÇºÃÆÀ  
maomao201142Â¥
2015-08-14 10:15   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
zhoujunbo43Â¥
2015-08-14 11:53   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
2015-08-14 12:41   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
2015-08-14 17:03   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
1314168apple46Â¥
2015-08-14 18:25   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
beijinghu47Â¥
2015-08-15 06:53   »Ø¸´  
ÎåÐÇºÃÆÀ  
duan198748Â¥
2015-08-15 11:40   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
gh_wang200849Â¥
2015-08-15 19:39   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
cy1302120250Â¥
2015-08-15 21:46   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
Ïà¹Ø°æ¿éÌø×ª ÎÒÒª¶©ÔÄÂ¥Ö÷ liygmail µÄÖ÷Ìâ¸üÐÂ
¡î ÎÞÐǼ¶ ¡ï Ò»ÐǼ¶ ¡ï¡ï¡ï ÈýÐǼ¶ ¡ï¡ï¡ï¡ï¡ï ÎåÐǼ¶
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] 085600 ²ÄÁÏÓ뻯¹¤ 329·ÖÇóµ÷¼Á +8 Mr. Z 2026-03-25 8/400 2026-03-25 23:00 by peike
[¿¼ÑÐ] ²ÄÁϵ÷¼Á 5+3 ÏëÒªÒ»ºøÌÒ»¨Ë® 2026-03-25 6/300 2026-03-25 18:20 by xcjcqu
[¿¼ÑÐ] 0854µç×ÓÐÅÏ¢Çóµ÷¼Á +7 ¦Á____ 2026-03-22 9/450 2026-03-25 13:37 by ¦Á____
[¿¼ÑÐ] 271Çóµ÷¼Á +4 ÉúÈçÏÄ»¨¡­ 2026-03-22 4/200 2026-03-25 11:25 by userper
[¿¼ÑÐ] 278Çóµ÷¼Á +4 ÎÒ¿ÉÒÔÉϰ¶µÄ¶Ô 2026-03-19 4/200 2026-03-25 11:01 by userper
[¿¼ÑÐ] Ò»Ö¾Ô¸ Î÷±±´óѧ £¬070300»¯Ñ§Ñ§Ë¶£¬×Ü·Ö287£¬Ë«·ÇÒ»±¾£¬Çóµ÷¼Á¡£ +4 ³¿»èÏßÓëÐǺ£ 2026-03-20 4/200 2026-03-25 10:16 by allen-yin
[¿¼ÑÐ] Ò»Ö¾Ô¸ÎäÀí085500»úеרҵ×Ü·Ö300Çóµ÷¼Á +3 an10101 2026-03-24 7/350 2026-03-25 00:00 by ɽ¹í0-
[Óлú½»Á÷] ÓлúºÏ³ÉÇóÖú 20+3 FENGSHUJEI 2026-03-23 5/250 2026-03-24 19:31 by 88817753
[¿¼ÑÐ] 291Çóµ÷¼Á +3 HanBeiNingZC 2026-03-24 3/150 2026-03-24 16:34 by barlinike
[¿¼ÑÐ] 307Çóµ÷¼Á +5 ³¬¼¶ÒÁ°º´óÍõ 2026-03-24 5/250 2026-03-24 15:46 by ÐÇ¿ÕÐÇÔÂ
[¿¼ÑÐ] 341Çóµ÷¼Á(Ò»Ö¾Ô¸ºþÄÏ´óѧ070300) +5 ·¬ÇÑÍ·--- 2026-03-22 6/300 2026-03-23 23:45 by Txy@872106
[¿¼ÑÐ] Ò»Ö¾Ô¸¹ú¿Æ¹ý³ÌËù081700£¬274Çóµ÷¼Á +3 ÈýË®ÑÐ0Ë®Á¢·½ 2026-03-23 3/150 2026-03-23 23:11 by MajorWen
[¿¼ÑÐ] 276Çóµ÷¼Á +3 YNRYG 2026-03-21 4/200 2026-03-23 08:31 by ×íÔÚ·çÀï
[¿¼ÑÐ] Ò»Ö¾Ô¸¶«»ª´óѧ»¯Ñ§070300£¬Çóµ÷¼Á +7 2117205181 2026-03-21 8/400 2026-03-22 22:55 by chixmc
[¿¼ÑÐ] ²ÄÁÏÓ뻯¹¤085600£¬×Ü·Ö304£¬±¾¿ÆÓÐÁ½Æªsci²ÎÓ룬Çóµ÷¼Á +4 ÐÒÔ˵Ľ´½´ 2026-03-22 5/250 2026-03-22 20:15 by edmund7
[¿¼ÑÐ] 324Çóµ÷¼Á +6 luckyѽѽѽѼ 2026-03-20 6/300 2026-03-22 16:01 by ColorlessPI
[¿¼ÑÐ] 303Çóµ÷¼Á +5 °²ÒäÁé 2026-03-22 6/300 2026-03-22 12:46 by ËØÑÕÇã³Ç1988
[¿¼ÑÐ] 085601µ÷¼Á 358·Ö +3 zzzzggh 2026-03-20 4/200 2026-03-21 10:21 by luoyongfeng
[¿¼ÑÐ] 288Çóµ÷¼Á +16 ÓÚº£º£º£º£ 2026-03-19 16/800 2026-03-20 22:28 by JourneyLucky
[¿¼ÑÐ] ²ÄÁÏѧ˶297ÒѹýËÄÁù¼¶Çóµ÷¼ÁÍÆ¼ö +11 adaie 2026-03-19 11/550 2026-03-20 21:30 by laoshidan
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û