²é¿´: 3644  |  »Ø¸´: 76
¡¾½±Àø¡¿ ±¾Ìû±»ÆÀ¼Û73´Î£¬×÷ÕßliygmailÔö¼Ó½ð±Ò 57.8 ¸ö

liygmail

ÖÁ×ðľ³æ (Ö°Òµ×÷¼Ò)


[×ÊÔ´] 2014ÄêÔ­°æµç×ÓÊé------DNA¿Ë¡ºÍ×é×°

2014ÄêÔ­°æµç×ÓÊé------DNA¿Ë¡ºÍ×é×°
ÄÚÈÝ£º
1 BioBrick Assembly Standards and Techniques ...
2  Sequence and Ligation-  Independent Cloning ......
3 Quick and Clean Cloning .............
4 Hierarchical Ligation-Independent Assembly of PCR Fragments ..........
6 Restriction-Free (RF) Cloning for Multicomponents Assembly ............... 73
7 A Single-Tube Assembly of DNA Using the Transfer-PCR (TPCR) Platform ................... 89
8 Circular Polymerase Extension Cloning ............................................................ 103
9 Golden Gate Cloning ....................................................................................... 119
10  GoldenBraid Cloning Strategy .................... 133
11 FX Cloning... 153
12 Minimum GC-Rich Sequences for Overlap Extension PCR and Primer Annealing ............ 165
...................
±¾·ÖÏí½öÓÃÓÚ½»Á÷ѧϰ£¬Èç¹û×ÊÔ´ÓÐÓã¬ÇëÓèÖÃÆÀ2014ÄêÔ­°æµç×ÓÊé------DNA¿Ë¡ºÍ×é×°
2015-08-08_124341.jpg
»Ø¸´´ËÂ¥

» ±¾Ìû¸½¼þ×ÊÔ´Áбí

  • »¶Ó­¼à¶½ºÍ·´À¡£ºÐ¡Ä¾³æ½öÌṩ½»Á÷ƽ̨£¬²»¶Ô¸ÃÄÚÈݸºÔð¡£
    ±¾ÄÚÈÝÓÉÓû§×ÔÖ÷·¢²¼£¬Èç¹ûÆäÄÚÈÝÉæ¼°µ½ÖªÊ¶²úȨÎÊÌ⣬ÆäÔðÈÎÔÚÓÚÓû§±¾ÈË£¬Èç¶Ô°æÈ¨ÓÐÒìÒ飬ÇëÁªÏµÓÊÏ䣺xiaomuchong@tal.com
  • ¸½¼þ 1 : DNA_Cloning_and_Assembly_Methods-2014.pdf
  • 2015-08-09 02:43:33, 9.01 M

» ÊÕ¼±¾ÌûµÄÌÔÌûר¼­ÍƼö

Êé¼®ÏÂÔØÍøÕ¾ רҵÊé¼®£¨ÍâÎİ棩WM ÉúÎïÒ½Ò©ÎÄÏ×Êé ÉúÎïҽѧ×ÊÁÏ
Biotech×ÊÁÏ ÄãÖµµÃÓµÓÐ ºÏ³ÉÉúÎïѧ

» ²ÂÄãϲ»¶

» ±¾Ö÷ÌâÏà¹Ø¼ÛÖµÌùÍÆ¼ö£¬¶ÔÄúͬÑùÓаïÖú:

ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

ÎÕ²»ÎÔ

гæ (³õÈëÎÄ̳)


¡ï¡ï¡ï¡ï¡ï ÎåÐǼ¶,ÓÅÐãÍÆ¼ö

顶一下,感谢分享�

[ ·¢×ÔСľ³æ¿Í»§¶Ë ]
8Â¥2015-08-10 01:24:58
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

zhangh62

ľ³æ (ÖøÃûдÊÖ)


¡ï¡ï¡ï¡ï¡ï ÎåÐǼ¶,ÓÅÐãÍÆ¼ö

ºÃÊ飬̫¸Ðл¥Ö÷À²£¡
9Â¥2015-08-10 11:08:56
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
¼òµ¥»Ø¸´
2015-08-09 07:26   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
jinhx873Â¥
2015-08-09 09:12   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
wzhsteve4Â¥
2015-08-09 13:19   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
xujian5685Â¥
2015-08-09 13:31   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
2015-08-09 17:27   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
1592364787Â¥
2015-08-09 17:57   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
lingshao40510Â¥
2015-08-10 11:22   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
VictoryDai11Â¥
2015-08-10 11:34   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
Goodman299512Â¥
2015-08-10 13:25   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
¶À¹Â²Ï13Â¥
2015-08-10 17:50   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
jianghy6914Â¥
2015-08-10 18:02   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
meilleur3315Â¥
2015-08-10 18:27   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
haozy8716Â¥
2015-08-10 23:01   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
shengdeqiao17Â¥
2015-08-11 09:24   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
daoke200518Â¥
2015-08-11 10:00   »Ø¸´  
ÈýÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
2015-08-11 10:31   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
maciter20Â¥
2015-08-11 10:58   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
lieying1821Â¥
2015-08-11 11:12   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
2015-08-11 11:13   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
zhchzhsh207623Â¥
2015-08-11 13:12   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
luntanhu24Â¥
2015-08-11 13:17   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
gogo25Â¥
2015-08-11 15:03   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
weishuang9826Â¥
2015-08-11 15:14   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
ha166827Â¥
2015-08-11 16:12   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
Q½À02128Â¥
2015-08-11 16:27   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
wildfire203829Â¥
2015-08-11 17:32   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
zhouxj30Â¥
2015-08-11 17:42   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
limingmaster31Â¥
2015-08-11 19:03   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
wangmingsh32Â¥
2015-08-11 21:22   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
biosensors33Â¥
2015-08-12 15:24   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
cellman34Â¥
2015-08-12 16:05   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
wmnick35Â¥
2015-08-12 21:25   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
2015-08-13 08:34   »Ø¸´  
¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
yuanchang37Â¥
2015-08-13 09:12   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
daiminzh38Â¥
2015-08-13 09:23   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
chieflee39Â¥
2015-08-13 14:40   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
lindoudou40Â¥
2015-08-13 17:19   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
everie41Â¥
2015-08-14 01:04   »Ø¸´  
ÎåÐÇºÃÆÀ  
maomao201142Â¥
2015-08-14 10:15   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
zhoujunbo43Â¥
2015-08-14 11:53   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
2015-08-14 12:41   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
2015-08-14 17:03   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
1314168apple46Â¥
2015-08-14 18:25   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
beijinghu47Â¥
2015-08-15 06:53   »Ø¸´  
ÎåÐÇºÃÆÀ  
duan198748Â¥
2015-08-15 11:40   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
gh_wang200849Â¥
2015-08-15 19:39   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
cy1302120250Â¥
2015-08-15 21:46   »Ø¸´  
ÎåÐÇºÃÆÀ  ¶¥Ò»Ï£¬¸Ðл·ÖÏí£¡
Ïà¹Ø°æ¿éÌø×ª ÎÒÒª¶©ÔÄÂ¥Ö÷ liygmail µÄÖ÷Ìâ¸üÐÂ
¡î ÎÞÐǼ¶ ¡ï Ò»ÐǼ¶ ¡ï¡ï¡ï ÈýÐǼ¶ ¡ï¡ï¡ï¡ï¡ï ÎåÐǼ¶
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] »¯Ñ§µ÷¼ÁÒ»Ö¾Ô¸ÉϺ£½»Í¨´óѧ336·Ö-±¾¿ÆÉϺ£211 +4 СÓã°®Óлú 2026-03-25 4/200 2026-03-26 10:19 by aa331100
[¿¼ÑÐ] ´ò¹ýºÜ¶à¾ºÈü£¬085406¿ØÖƹ¤³Ì300·Ö£¬Çóµ÷¼Á +3 askeladz 2026-03-26 3/150 2026-03-26 09:08 by ¸øÄãÄã×¢ÒâÐÝÏ¢
[¿¼ÑÐ] Ò»Ö¾Ô¸¹þ¹¤´ó£¬085400£¬320£¬Çóµ÷¼Á +4 gdlf9999 2026-03-24 4/200 2026-03-25 23:01 by boxking200
[¿¼ÑÐ] ²ÄÁÏÓ뻯¹¤304ÇóBÇøµ÷¼Á +3 Çñgl 2026-03-25 3/150 2026-03-25 19:03 by Ainin_
[¿¼ÑÐ] ²ÄÁÏÓ뻯¹¤ 322Çóµ÷¼Á +6 È»11 2026-03-19 6/300 2026-03-25 18:37 by haxia
[¿¼ÑÐ] ¿¼ÑÐÒ»Ö¾Ô¸ËÕÖÝ´óѧ³õʼ315£¨Ó¢Ò»£©Çóµ÷¼Á +3 sbdksD 2026-03-24 4/200 2026-03-25 18:16 by xcjcqu
[¿¼ÑÐ] ÍøÂç¿Õ¼ä°²È«0839Õе÷¼Á +4 w320357296 2026-03-25 6/300 2026-03-25 17:59 by 255671
[¿¼ÑÐ] 284Çóµ÷¼Á +15 Zhao anqi 2026-03-22 15/750 2026-03-25 12:51 by wht0531
[¿¼ÑÐ] ¿¼Ñл¯Ñ§308·ÖÇóµ÷¼Á +10 ÄãºÃÃ÷ÌìÄãºÃ 2026-03-23 11/550 2026-03-25 10:23 by userper
[¿¼ÑÐ] BÇø¿¼Ñе÷¼Á +4 yqdszhdap£­ 2026-03-22 5/250 2026-03-25 08:51 by baoball
[¿¼ÑÐ] 0703»¯Ñ§µ÷¼Á£¬Çóµ¼Ê¦ÊÕ +7 ÌìÌìºÃÔËÀ´Éϰ¶° 2026-03-24 7/350 2026-03-24 20:26 by peike
[¿¼ÑÐ] 300Çóµ÷¼Á£¬²ÄÁÏ¿ÆÑ§Ó¢Ò»Êý¶þ +5 leaflight 2026-03-24 5/250 2026-03-24 16:25 by laoshidan
[¿¼ÑÐ] »¯Ñ§308·ÖÇóµ÷¼Á +3 ÄãºÃÃ÷ÌìÄãºÃ 2026-03-23 3/150 2026-03-23 20:11 by macy2011
[¿¼ÑÐ] 328Çóµ÷¼Á +4 LHHL66 2026-03-23 4/200 2026-03-23 14:55 by lbsjt
[¿¼ÑÐ] 293Çóµ÷¼Á +3 ÌÎÌÎWjt 2026-03-22 5/250 2026-03-22 22:21 by jiangpengfei
[¿¼ÑÐ] Ò»Ö¾Ô¸ÖØÇì´óѧ085700×ÊÔ´Óë»·¾³×Ü·Ö308Çóµ÷¼Á +7 īīĮ 2026-03-20 7/350 2026-03-21 16:36 by barlinike
[¿¼ÑÐ] Çóµ÷¼Á +3 .m.. 2026-03-21 4/200 2026-03-21 16:25 by barlinike
[¿¼ÑÐ] 22408 344·Ö Çóµ÷¼Á Ò»Ö¾Ô¸ »ªµç¼ÆËã»ú¼¼Êõ +4 solanXXX 2026-03-20 4/200 2026-03-20 23:49 by alg094825
[¿¼ÑÐ] AÇøÏß²ÄÁÏѧµ÷¼Á +5 ÖÜÖÜÎÞ¼« 2026-03-20 5/250 2026-03-20 21:33 by laoshidan
[¿¼ÑÐ] Ò»Ö¾Ô¸Î÷°²½»Í¨´óѧ ѧ˶ 354Çóµ÷¼Á211»òÕß˫һÁ÷ +3 ÎÒÏëÒª¶ÁÑо¿Éú 2026-03-20 3/150 2026-03-20 20:13 by JourneyLucky
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û