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As DHT is a weak acid substances, adding a certain amount of acid to   the mobile phase for HPLC analysis can reduce the tailing of the peak and increase the symmetry of the peak. In the experiments formic acid and acetic acid were used. The effects of formic acid and acetic acid on the separation of DHT enantiomers were listed in Table 3. µ±Á÷¶¯ÏàÖв»Ìí¼Ó¼×ËáºÍÒÒËáʱ£¬DHTµÄÁ½¸ö¶ÔÓ³Ìå¿ÉÒÔ´ïµ½»ùÏß·ÖÀë¡£¼øÓÚÖù×ÓµÄÄÍÓÃÐÔ£¬Á÷¶¯ÏàÖÐËáÌí¼ÓÁ¿ÔÚ 0. 1% ~ 0.2% Ö®¼äʱ£¬ DHT¶ÔÓ³Ìå¿ÉͬʱʵÏÖ»ùÏß·ÖÀë¡£ Ϊ»ñµÃ½ÏºÃµÄ·ÖÀëЧ¹û£¬²¢¾¡Á¿½ÏÉÙ±£Áôʱ¼ä£¬×îÖÕÑ¡ÔñÌí¼Ó0.2%¼×Ëá¡£When the separation was conducted using a mobile phase without formic acid and acetic acid, a baseline separation could (not?) be achieved for the two enantiomers of DHT. In view of not to affect the durability of the column, when the acid content was between 0.1% ~ 0.2% in the mobile phase, DHT enantiomers could simultaneously achieve baseline separation.  To achieve better separation results, and to reduce the retention time as short as possible, finally we chose to add 0.2% formic acid.
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jiushengming: ½ð±Ò+20, ·­ÒëEPI+1, ¡ï¡ï¡ïºÜÓаïÖú, ллÄúµÄ°ïÖú£¡ 2015-05-31 12:51:11
DHT was a weak acidic material. Adding  certain amount of acid (formic acid and acetic acid were used in this experiment) into mobile phase in HPLC analysis chould reduce tailed chromatographic peaks and increase its degree of symmetry.
The effects of the additive amount of formic acid and acetic acid in  mobile phase to DHT enantiomeric separation were listed in table 3.
The two DHT enantiomorphs chould  achieve baseline separation, without no acid(formic acid and acetic acid) addition in mobile phase.
Considering the durability of column, DHT enantiomorphs were  simultaneous  achieved baseline separation when acid addition was  
0. 1% ~ 0.2%. Furthermore, 0.2% formic acid  addition was final chose to obtain better separation effect and less retention time simultaneously.
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