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jiushengming: 金币+20, 翻译EPI+1, ★★★很有帮助, 谢谢您的帮助！ 2015-05-31 12:51:11

DHT was a weak acidic material. Adding  certain amount of acid (formic acid and acetic acid were used in this experiment) into mobile phase in HPLC analysis chould reduce tailed chromatographic peaks and increase its degree of symmetry.
The effects of the additive amount of formic acid and acetic acid in  mobile phase to DHT enantiomeric separation were listed in table 3.
The two DHT enantiomorphs chould  achieve baseline separation, without no acid(formic acid and acetic acid) addition in mobile phase.
Considering the durability of column, DHT enantiomorphs were  simultaneous  achieved baseline separation when acid addition was
0. 1% ~ 0.2%. Furthermore, 0.2% formic acid  addition was final chose to obtain better separation effect and less retention time simultaneously.

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