至尊木虫 (知名作家)
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【答案】应助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ... 永不停息的风: 金币+200, ★★★★★最佳答案 2015-05-17 11:09:30 RXMCDM: 翻译EPI+1 2015-05-17 19:58:39
该论文摘要翻译如下(给你翻译是因为对内容熟悉):
冬凌草甲素改变人胰腺癌细胞BxPC-3 MicroRNA的表达谱
摘要
背景:作为中国传统医学中使用的一种成分,已经证明冬凌草甲素具有重要的抗肿瘤作用,但其抗肿瘤特性的机理还不清楚。
方法:为验证冬凌草甲素的抗癌作用是通过一种miRNA的依赖机制,用冬凌草甲素处理BxPC-3人胰腺癌细胞,根据Sanger miR-Base 20版本用miRNA芯片实验对miRNA的综合表达谱进行分析,随后用实时PCR予以验证。并预测了microRNA的靶基因、分析研究了基因本体和KEGG通路,以研究可能参与的信号通路。
结果:结果表明,在冬凌草甲素处理的BxPC-3人胰腺癌细胞中,有105种miRNA表达呈现显著差异(信号读数>500,p≤0.01,| Log2-值|≥1)。
结论:
我们的数据表明,冬凌草甲素抑制BxPC-3细胞可能是通过调节miRNA的表达。干扰miRNA的表达谱可能会为临床治疗胰腺癌提供一种新的治疗方法。
附:原文摘要
Oridonin alters the expression profiles of MicroRNAs in BxPC-3 human pancreatic cancer cells.
Abstract
BACKGROUND: Oridonin, an ingredient used in traditional Chinese medicine, has been demonstrated to play an important role in antitumour effects, but the mechanism underlying its antitumour properties is still not clear.
METHODS:To verify the anti-cancer effects of oridonin via a miRNA-dependent mechanism, comprehensive miRNA expression profiling of oridonin-treated BxPC-3 human pancreatic cancer cells was performed using a miRNA microarray assay based on Sanger miR-Base Release 20, followed by a validation using real-time PCR. MicroRNA target prediction and Gene Ontology and KEGG pathway analysis were performed to investigate possible pathways involved.
RESULTS:The results showed that 105 miRNAs were significantly differentially expressed (signal reading >500, p ≤ 0.01, |Log2-value| ≥1) in oridonin-treated BxPC-3 human pancreatic cancer cells.
CONCLUSIONS: Our data indicates that oridonin inhibits BxPC-3 cells probably through regulating the expression of miRNAs. Interruption of miRNA profiling may provide new therapeutic methods for the clinical treatment of pancreatic cancer. |
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