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2楼2015-05-17 03:26:54
ssssllllnnnn
至尊木虫 (知名作家)
Translator and Proofreader
- 翻译EPI: 1690
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永不停息的风: 金币+200, ★★★★★最佳答案 2015-05-17 11:09:30
RXMCDM: 翻译EPI+1 2015-05-17 19:58:39
永不停息的风: 金币+200, ★★★★★最佳答案 2015-05-17 11:09:30
RXMCDM: 翻译EPI+1 2015-05-17 19:58:39
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该论文摘要翻译如下(给你翻译是因为对内容熟悉): 冬凌草甲素改变人胰腺癌细胞BxPC-3 MicroRNA的表达谱 摘要 背景:作为中国传统医学中使用的一种成分,已经证明冬凌草甲素具有重要的抗肿瘤作用,但其抗肿瘤特性的机理还不清楚。 方法:为验证冬凌草甲素的抗癌作用是通过一种miRNA的依赖机制,用冬凌草甲素处理BxPC-3人胰腺癌细胞,根据Sanger miR-Base 20版本用miRNA芯片实验对miRNA的综合表达谱进行分析,随后用实时PCR予以验证。并预测了microRNA的靶基因、分析研究了基因本体和KEGG通路,以研究可能参与的信号通路。 结果:结果表明,在冬凌草甲素处理的BxPC-3人胰腺癌细胞中,有105种miRNA表达呈现显著差异(信号读数>500,p≤0.01,| Log2-值|≥1)。 结论: 我们的数据表明,冬凌草甲素抑制BxPC-3细胞可能是通过调节miRNA的表达。干扰miRNA的表达谱可能会为临床治疗胰腺癌提供一种新的治疗方法。 附:原文摘要 Oridonin alters the expression profiles of MicroRNAs in BxPC-3 human pancreatic cancer cells. Abstract BACKGROUND: Oridonin, an ingredient used in traditional Chinese medicine, has been demonstrated to play an important role in antitumour effects, but the mechanism underlying its antitumour properties is still not clear. METHODS:To verify the anti-cancer effects of oridonin via a miRNA-dependent mechanism, comprehensive miRNA expression profiling of oridonin-treated BxPC-3 human pancreatic cancer cells was performed using a miRNA microarray assay based on Sanger miR-Base Release 20, followed by a validation using real-time PCR. MicroRNA target prediction and Gene Ontology and KEGG pathway analysis were performed to investigate possible pathways involved. RESULTS:The results showed that 105 miRNAs were significantly differentially expressed (signal reading >500, p ≤ 0.01, |Log2-value| ≥1) in oridonin-treated BxPC-3 human pancreatic cancer cells. CONCLUSIONS: Our data indicates that oridonin inhibits BxPC-3 cells probably through regulating the expression of miRNAs. Interruption of miRNA profiling may provide new therapeutic methods for the clinical treatment of pancreatic cancer. |
3楼2015-05-17 04:44:43
4楼2015-05-17 10:17:13
fjtony163
版主 (文坛精英)
米米
- 翻译EPI: 386
- 应助: 361 (硕士)
- 贵宾: 6.876
- 金币: 70614.6
- 散金: 36050
- 红花: 222
- 沙发: 616
- 帖子: 21912
- 在线: 3793.7小时
- 虫号: 1397876
- 注册: 2011-09-11
- 专业: 遗传学研究新技术与方法
- 管辖: 论文翻译
5楼2015-05-17 10:30:37
6楼2015-05-17 10:46:59
fjtony163
版主 (文坛精英)
米米
- 翻译EPI: 386
- 应助: 361 (硕士)
- 贵宾: 6.876
- 金币: 70614.6
- 散金: 36050
- 红花: 222
- 沙发: 616
- 帖子: 21912
- 在线: 3793.7小时
- 虫号: 1397876
- 注册: 2011-09-11
- 专业: 遗传学研究新技术与方法
- 管辖: 论文翻译
7楼2015-05-17 12:49:49
8楼2015-05-17 14:51:25













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