|
|
【答案】应助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★
感谢参与，应助指数 +1
icebergwu: 金币+10, ★★★★★最佳答案 2015-04-28 20:33:17
sunshan4379: LS-EPI+1, 感谢应助！ 2015-04-28 20:58:55
Bioconversion, Purification and In Vitro Antibacterial Activity of Platenomycin A1
作者:Wu, JX (Wu, Jiaxin)[ 1,2,3,4 ] ; Zhang, GD (Zhang, Guodong)[ 1,2,3,4 ] ; Song, M (Song, Min)[ 1,2,3,4 ] ; Qi, P (Qi, Peng)[ 1,2,3,4 ] ; He, JH (He, Jihong)[ 1,2,3,4 ] ; Zheng, YH (Zheng, Yinghua)[ 1,2,3,4 ] ; Zhao, MX (Zhao, Meixian)[ 1,2,3,4 ] ; Ge, XM (Ge, XinMei)[ 1,2,3,4 ] ; Cao, Q (Cao, Qin)[ 5 ]
LATIN AMERICAN JOURNAL OF PHARMACY
卷: 33 期: 9 页: 1574-1578
出版年: 2014
查看期刊信息
摘要
Platenomycin A1 is one type of 16-membered macrolide antibiotics, which had been shown to inhibit the Gram-positive bacteria growth. In this study, the bioconversion of platenomycin A1 from midecamycin in Streptomyces thermotolerans was investigated. With optimized growth conditions, platenomycin A1 production reached to 5.654 g/L. In addition, the platenomycin A1 separation and purification procedures were developed based on pH adjustment and ODS reversed phase chromatography refinement, which leaded to a purity up to 97.74%. Minimum inhibitory concentrations (MIC) of purified platenomycin A1 against representive gram-positive and gram-negative bacteria strains were also determined, which displayed active inhibition against Staphyloccocus aureus (ATCC29213), Streptococcus pneumonia (ATCC49619), septic Streptococcus (CVCC593) and porcine Actinobacillus pleuropneumoniae (CVCC260).
关键词
作者关键词:Antibacterial activity; Bioconversion; Midecamycin; Platenomycin A1; purification
KeyWords Plus:ANTIBIOTIC YL-704 COMPLEX; STREPTOMYCES-THERMOTOLERANS; PHYSICOCHEMICAL PROPERTIES; MACROLIDE ANTIBIOTICS; ACYL DERIVATIVES; MIDECAMYCIN; TYLOSIN
作者信息
通讯作者地址: Wu, JX (通讯作者)
China Anim Husb Ind Co Ltd, Beijing 100095, Peoples R China.
地址:
[ 1 ] China Anim Husb Ind Co Ltd, Beijing 100095, Peoples R China
[ 2 ] Minist Agr, Key Lab Biol Prod & Chem Drugs Anim, Beijing 100095, Peoples R China
[ 3 ] Beijing Engn Res Ctr Design & Dev Synthet Peptide, Beijing 100095, Peoples R China
[ 4 ] China Anim Husb Grp, Beijing 100095, Peoples R China
[ 5 ] Chinese Natl Ctr Biotechnol Dev, Beijing 100039, Peoples R China
电子邮件地址:icebergwujiaxin@163.com
基金资助致谢
基金资助机构 授权号
National High Technology Research and Development Program of China (863 Program)
2011AA10A214
查看基金资助信息
出版商
COLEGIO FARMACEUTICOS PROVINCIA DE BUENOS AIRES, DEPT CIENTIFICO, CALLE 5 NO 966, LA PLATA, 00000, ARGENTINA
类别 / 分类
研究方向 harmacology & Pharmacy
Web of Science 类别harmacology & Pharmacy
文献信息
文献类型:Article
语种:English
入藏号: WOS:000347782100027
ISSN: 0326-2383
期刊信息
Impact Factor (影响因子): Journal Citation Reports®
其他信息
IDS 号: AY8CM
Web of Science 核心合集中的 "引用的参考文献": 16
Web of Science 核心合集中的 "被引频次": 0 |
|
回复此楼