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Çë¸÷λ³æÓѰïæ°ïæ¼ìË÷һϣ¬¿´¿´Bioconversion, purification and in vitro antibacterial activity of Platenomycin A1ÊDz»ÊÇÒѾ­±»SCIÊÕ¼ÁË£¬¸ÃÎÄÕ·¢±íÓÚLatin American Journal of Pharmacy£¬33 (9): 1574-8 (2014)

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icebergwu: ½ð±Ò+10, ¡ï¡ï¡ï¡ï¡ï×î¼Ñ´ð°¸ 2015-04-28 20:33:17
sunshan4379: LS-EPI+1, ¸ÐлӦÖú£¡ 2015-04-28 20:58:55
Bioconversion, Purification and In Vitro Antibacterial Activity of Platenomycin A1
×÷Õß:Wu, JX (Wu, Jiaxin)[ 1,2,3,4 ] ; Zhang, GD (Zhang, Guodong)[ 1,2,3,4 ] ; Song, M (Song, Min)[ 1,2,3,4 ] ; Qi, P (Qi, Peng)[ 1,2,3,4 ] ; He, JH (He, Jihong)[ 1,2,3,4 ] ; Zheng, YH (Zheng, Yinghua)[ 1,2,3,4 ] ; Zhao, MX (Zhao, Meixian)[ 1,2,3,4 ] ; Ge, XM (Ge, XinMei)[ 1,2,3,4 ] ; Cao, Q (Cao, Qin)[ 5 ]
LATIN AMERICAN JOURNAL OF PHARMACY
¾í: 33  ÆÚ: 9  Ò³: 1574-1578
³ö°æÄê: 2014
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Platenomycin A1 is one type of 16-membered macrolide antibiotics, which had been shown to inhibit the Gram-positive bacteria growth. In this study, the bioconversion of platenomycin A1 from midecamycin in Streptomyces thermotolerans was investigated. With optimized growth conditions, platenomycin A1 production reached to 5.654 g/L. In addition, the platenomycin A1 separation and purification procedures were developed based on pH adjustment and ODS reversed phase chromatography refinement, which leaded to a purity up to 97.74%. Minimum inhibitory concentrations (MIC) of purified platenomycin A1 against representive gram-positive and gram-negative bacteria strains were also determined, which displayed active inhibition against Staphyloccocus aureus (ATCC29213), Streptococcus pneumonia (ATCC49619), septic Streptococcus (CVCC593) and porcine Actinobacillus pleuropneumoniae (CVCC260).
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×÷Õ߹ؼü´Ê:Antibacterial activity; Bioconversion; Midecamycin; Platenomycin A1; purification
KeyWords Plus:ANTIBIOTIC YL-704 COMPLEX; STREPTOMYCES-THERMOTOLERANS; PHYSICOCHEMICAL PROPERTIES; MACROLIDE ANTIBIOTICS; ACYL DERIVATIVES; MIDECAMYCIN; TYLOSIN
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ͨѶ×÷ÕßµØÖ·: Wu, JX (ͨѶ×÷Õß)
              China Anim Husb Ind Co Ltd, Beijing 100095, Peoples R China.
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              [ 1 ] China Anim Husb Ind Co Ltd, Beijing 100095, Peoples R China
              [ 2 ] Minist Agr, Key Lab Biol Prod & Chem Drugs Anim, Beijing 100095, Peoples R China
              [ 3 ] Beijing Engn Res Ctr Design & Dev Synthet Peptide, Beijing 100095, Peoples R China
              [ 4 ] China Anim Husb Grp, Beijing 100095, Peoples R China
              [ 5 ] Chinese Natl Ctr Biotechnol Dev, Beijing 100039, Peoples R China
µç×ÓÓʼþµØÖ·:icebergwujiaxin@163.com
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National High Technology Research and Development Program of China (863 Program)        
2011AA10A214
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COLEGIO FARMACEUTICOS PROVINCIA DE BUENOS AIRES, DEPT CIENTIFICO, CALLE 5 NO 966, LA PLATA, 00000, ARGENTINA
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Èë²ØºÅ: WOS:000347782100027
ISSN: 0326-2383
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icebergwu

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2Â¥: Originally posted by Ðľ²_ÒÀÈ» at 2015-04-28 19:57:41
Bioconversion, Purification and In Vitro Antibacterial Activity of Platenomycin A1
×÷Õß:Wu, JX (Wu, Jiaxin) ; Zhang, GD (Zhang, Guodong) ; Song, M (Song, Min) ; Qi, P (Qi, Peng) ; He, JH (He, Jihong ...

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3Â¥: Originally posted by icebergwu at 2015-04-28 20:34:09
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4Â¥: Originally posted by Ðľ²_ÒÀÈ» at 2015-04-28 21:46:56
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