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| Çë¸÷λ³æÓѰïæ°ïæ¼ìË÷һϣ¬¿´¿´Bioconversion, purification and in vitro antibacterial activity of Platenomycin A1ÊDz»ÊÇÒѾ±»SCIÊÕ¼ÁË£¬¸ÃÎÄÕ·¢±íÓÚLatin American Journal of Pharmacy£¬33 (9): 1574-8 (2014) |
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icebergwu: ½ð±Ò+10, ¡ï¡ï¡ï¡ï¡ï×î¼Ñ´ð°¸ 2015-04-28 20:33:17
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icebergwu: ½ð±Ò+10, ¡ï¡ï¡ï¡ï¡ï×î¼Ñ´ð°¸ 2015-04-28 20:33:17
sunshan4379: LS-EPI+1, ¸ÐлӦÖú£¡ 2015-04-28 20:58:55
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Bioconversion, Purification and In Vitro Antibacterial Activity of Platenomycin A1 ×÷Õß:Wu, JX (Wu, Jiaxin)[ 1,2,3,4 ] ; Zhang, GD (Zhang, Guodong)[ 1,2,3,4 ] ; Song, M (Song, Min)[ 1,2,3,4 ] ; Qi, P (Qi, Peng)[ 1,2,3,4 ] ; He, JH (He, Jihong)[ 1,2,3,4 ] ; Zheng, YH (Zheng, Yinghua)[ 1,2,3,4 ] ; Zhao, MX (Zhao, Meixian)[ 1,2,3,4 ] ; Ge, XM (Ge, XinMei)[ 1,2,3,4 ] ; Cao, Q (Cao, Qin)[ 5 ] LATIN AMERICAN JOURNAL OF PHARMACY ¾í: 33 ÆÚ: 9 Ò³: 1574-1578 ³ö°æÄê: 2014 ²é¿´ÆÚ¿¯ÐÅÏ¢ ÕªÒª Platenomycin A1 is one type of 16-membered macrolide antibiotics, which had been shown to inhibit the Gram-positive bacteria growth. In this study, the bioconversion of platenomycin A1 from midecamycin in Streptomyces thermotolerans was investigated. With optimized growth conditions, platenomycin A1 production reached to 5.654 g/L. In addition, the platenomycin A1 separation and purification procedures were developed based on pH adjustment and ODS reversed phase chromatography refinement, which leaded to a purity up to 97.74%. Minimum inhibitory concentrations (MIC) of purified platenomycin A1 against representive gram-positive and gram-negative bacteria strains were also determined, which displayed active inhibition against Staphyloccocus aureus (ATCC29213), Streptococcus pneumonia (ATCC49619), septic Streptococcus (CVCC593) and porcine Actinobacillus pleuropneumoniae (CVCC260). ¹Ø¼ü´Ê ×÷Õ߹ؼü´Ê:Antibacterial activity; Bioconversion; Midecamycin; Platenomycin A1; purification KeyWords Plus:ANTIBIOTIC YL-704 COMPLEX; STREPTOMYCES-THERMOTOLERANS; PHYSICOCHEMICAL PROPERTIES; MACROLIDE ANTIBIOTICS; ACYL DERIVATIVES; MIDECAMYCIN; TYLOSIN ×÷ÕßÐÅÏ¢ ͨѶ×÷ÕßµØÖ·: Wu, JX (ͨѶ×÷Õß) China Anim Husb Ind Co Ltd, Beijing 100095, Peoples R China. µØÖ·: [ 1 ] China Anim Husb Ind Co Ltd, Beijing 100095, Peoples R China [ 2 ] Minist Agr, Key Lab Biol Prod & Chem Drugs Anim, Beijing 100095, Peoples R China [ 3 ] Beijing Engn Res Ctr Design & Dev Synthet Peptide, Beijing 100095, Peoples R China [ 4 ] China Anim Husb Grp, Beijing 100095, Peoples R China [ 5 ] Chinese Natl Ctr Biotechnol Dev, Beijing 100039, Peoples R China µç×ÓÓʼþµØÖ·:icebergwujiaxin@163.com »ù½ð×ÊÖúÖÂл »ù½ð×ÊÖú»ú¹¹ ÊÚȨºÅ National High Technology Research and Development Program of China (863 Program) 2011AA10A214 ²é¿´»ù½ð×ÊÖúÐÅÏ¢ ³ö°æÉÌ COLEGIO FARMACEUTICOS PROVINCIA DE BUENOS AIRES, DEPT CIENTIFICO, CALLE 5 NO 966, LA PLATA, 00000, ARGENTINA Àà±ð / ·ÖÀà Ñо¿·½Ïò harmacology & PharmacyWeb of Science Àà±ð harmacology & PharmacyÎÄÏ×ÐÅÏ¢ ÎÄÏ×ÀàÐÍ:Article ÓïÖÖ:English Èë²ØºÅ: WOS:000347782100027 ISSN: 0326-2383 ÆÚ¿¯ÐÅÏ¢ Impact Factor (Ó°ÏìÒò×Ó): Journal Citation Reports® ÆäËûÐÅÏ¢ IDS ºÅ: AY8CM Web of Science ºËÐĺϼ¯ÖÐµÄ "ÒýÓõIJο¼ÎÄÏ×": 16 Web of Science ºËÐĺϼ¯ÖÐµÄ "±»ÒýƵ´Î": 0 |

2Â¥2015-04-28 19:57:41
icebergwu
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3Â¥2015-04-28 20:34:09
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4Â¥2015-04-28 21:46:56
icebergwu
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5Â¥2015-04-28 22:10:48













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harmacology & Pharmacy