±±¾©Ê¯ÓÍ»¯¹¤Ñ§Ôº2026ÄêÑо¿ÉúÕÐÉú½ÓÊÕµ÷¼Á¹«¸æ
²é¿´: 186  |  »Ø¸´: 2
µ±Ç°Ö÷ÌâÒѾ­´æµµ¡£
µ±Ç°Ö»ÏÔʾÂú×ãÖ¸¶¨Ìõ¼þµÄ»ØÌû£¬µã»÷ÕâÀï²é¿´±¾»°ÌâµÄËùÓлØÌû

xiaricha

Òø³æ (СÓÐÃûÆø)

[½»Á÷] ʵÑéûÓнá¹û£¬Çë¸ßÊÖÖ¸µã£¡£¨rt-pcr£©

¸÷λսÓÑ£ºÎÒ×öʵÑéÓöµ½Á˺ܼ¬ÊÖµÄÎÊÌ⣬ϣÍû¸ßÊֵ㲦һÏ¡£
ÎÒÌáµÄRNAÒѾ­¼ì²â¹ýÁË£¬Ó¦¸ÃûÓÐÎÊÌâ¡£·´×ªÂ¼ÌåϵÈçÏ£ºRNA 3UL,OLIGOD(T) 1 UL, DTT 1UL,µÚÒ»Á´ºÏ³ÉBUFFER 2ul £¬0.5ulµÄDNTP£¬0.5ulRI ,0.5ulSuper SCRIPTII ·´×ªÂ¼Ã¸ £¬²¹Ë®ÖÁ10ul¡£ÔÚ×ö·´×ªÂ¼Ç°È¡2ulRNA£¬65¶Èˮԡ5·ÖÖÓÈ»ºó±ùÔ£¡£½ÓÏÂÀ´PCR¡£Õâ¸öÌåϵÓÐÎÊÌâÂð£¿
ÉÏÓÎ:CCCACACAGTCGCTCCATAG 57.8¶È
ÏÂÓÎ:AATCCTCGGTCCTTGCGTAG 57.9¶È   PCR·´Ó¦ÌåϵÈçÏ£º
1£º10Xbuffer:2.5UL
2£ºÒýÎï¸÷20UM 0.5uL
3£ºmgcl2(25mM)1.5uL
4£ºdNTP(2.5mM)4ul
5£ºÄ£°å¼Ó1ulcDNA.
6£ºrTaqø0.5ul£¬
7£º×îºó²¹Ë®ÖÁ25uL.
·´Ó¦Ìõ¼þΪ:
1: 94¶È3·ÖÖÓ,
2: 94¶È30Ãë
3£º£¨52-62£©¶È30Ãë
4:72¶È2·ÖÖÓ
5:ÖØ¸´2-4 33µ½35¸öÑ­»·
6:72¶È10·ÖÖÓ
¿ÉΪʲôһֱ²»³öÌõ´ø°¡£¿ÕæµÄºÜ׿±°¡£¡µ½µ×ÊÇÄĸöµØ·½³öÁËÎÊÌâ°¡£¿
»Ø¸´´ËÂ¥

» ²ÂÄãϲ»¶

ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

Kiddy

½ð³æ (СÓÐÃûÆø)

¡ï
lixiaoyi1981(½ð±Ò+1,VIP+0):Õâ¸ö½¨Òé²»´í
ÔÚ×ö·´×ªÂ¼Ç°È¡2ulRNA£¬65¶Èˮԡ5·ÖÖÓÈ»ºó±ùÔ£¡£½ÓÏÂÀ´PCR
ÕâÊÇʲôÒâ˼£¿°ÑRNAˮԡÂð£¿
ÏȽ«RNA\DNTP\OLIGODT\0.1DTT»ìºÏºó65¶È5·ÖÖÓ£¬±ùÔ¡2-3·ÖÖÓ£¬ÔÙ¼ÌÐø¼ÓÈëʣϵÄÈÜÒººÍø£¡ÕâÑùÓ¦¸ÃÊÇûÎÊÌâµÄ
Äã¿ÉÒÔÏÈ×ö¸öÄڲΣ¬ÔÙpcr×Ô¼ºÒªµÄÄ¿µÄƬ¶Ï
3Â¥2008-06-27 10:12:09
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
²é¿´È«²¿ 3 ¸ö»Ø´ð

yangfang072

гæ (³õÈëÎÄ̳)

ÔËÆø

ÎÒ¾õµÃ×ö·Ö×ÓÓÀÔ¶ºÜ¹îÒì
Ã÷Ã÷È«²¿¶¼ÕýÈ·µ«¾ÍµÃ²»µ½
Äã¼ÌÐøÅ¬Á¦
¼ÓÓÍ
ÔËÆøµ½Á˾ͺÃÁË
2Â¥2008-06-27 09:28:40
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] 289Çóµ÷¼Á +9 ÐÂʱ´ú²ÄÁÏ 2026-03-27 9/450 2026-03-28 09:28 by baoball
[¿¼ÑÐ] 085405 ¿¼µÄ11408Çó¸÷λÀÏʦ´ø×ß +3 Qiuѧing 2026-03-28 3/150 2026-03-28 09:19 by ÀֺǺǵÄ×·ÃÎÈË
[¿¼ÑÐ] 0703»¯Ñ§/290Çóµ÷¼Á/±¾¿Æ¾­Àú·á¸»/¹¤¿ÆÒ²¿É +9 µ¤ÇàÄÌ¸Ç 2026-03-26 10/500 2026-03-28 07:45 by barnett0632
[¿¼ÑÐ] 0856²ÄÁÏ»¯¹¤µ÷¼Á ×Ü·Ö330 +10 zhubinhao 2026-03-27 10/500 2026-03-28 03:34 by fmesaito
[¿¼ÑÐ] 299Çóµ÷¼Á +6 àÅàÅàÅàÅ2 2026-03-27 6/300 2026-03-27 12:43 by ¹û¹ûÂèßä
[¿¼ÑÐ] 081200-11408-276ѧ˶Çóµ÷¼Á +3 ´Þwj 2026-03-26 3/150 2026-03-27 07:58 by chemisry
[¿¼ÑÐ] ¡¾Ë«Ò»Á÷ԺУÐÂÄÜÔ´¡¢»·¾³²ÄÁÏ£¬²ÄÁϼӹ¤ÓëÄ£ÄâÕÐÊÕ´óÁ¿µ÷¼Á¡¿ +4 Higraduate 2026-03-22 8/400 2026-03-26 20:34 by Higraduate
[¿¼ÑÐ] 085602 289·ÖÇóµ÷¼Á +8 WWWÎ÷Î÷¸¥Ë¹ 2026-03-24 8/400 2026-03-26 16:33 by ²»³Ôô~µÄ؈
[¿¼ÑÐ] 085601Çóµ÷¼Á×Ü·Ö293Ó¢Ò»Êý¶þ +4 ¸ÖÌú´óÅÚ 2026-03-24 4/200 2026-03-26 16:28 by dick_runner
[¿¼ÑÐ] 289Çóµ÷¼Á +17 ˶ÐǸ° 2026-03-23 17/850 2026-03-26 16:18 by ²»³Ôô~µÄ؈
[¿¼ÑÐ] 334·Ö Ò»Ö¾Ô¸ÎäÀí ²ÄÁÏÇóµ÷¼Á +4 ÀîÀî²»·þÊä 2026-03-26 4/200 2026-03-26 16:00 by ²»³Ôô~µÄ؈
[¿¼ÑÐ] 086000ÉúÎïÓëÒ½Ò©292Çóµ÷¼Á +6 СС³ÂСС 2026-03-22 9/450 2026-03-26 15:58 by dick_runner
[¿¼ÑÐ] 07»¯Ñ§303Çóµ÷¼Á +5 î£08 2026-03-25 5/250 2026-03-25 22:46 by 418490947
[¿¼ÑÐ] ²ÄÁÏÓ뻯¹¤328·Öµ÷¼Á +6 ¡££¬¡££¬¡££¬¡£i 2026-03-23 6/300 2026-03-25 22:30 by 418490947
[¿¼ÑÐ] 334·Ö Ò»Ö¾Ô¸ÎäÀí-080500 ²ÄÁÏÇóµ÷¼Á +4 ÀîÀî²»·þÊä 2026-03-25 4/200 2026-03-25 21:26 by ÐÇ¿ÕÐÇÔÂ
[¿¼ÑÐ] 0854È˹¤ÖÇÄÜ·½ÏòÕÐÊÕµ÷¼Á +4 ÕÂСÓã567 2026-03-24 4/200 2026-03-25 13:29 by 2177681040
[¿¼ÑÐ] BÇø¿¼Ñе÷¼Á +4 yqdszhdap£­ 2026-03-22 5/250 2026-03-25 08:51 by baoball
[¿¼ÑÐ] 292Çóµ÷¼Á +4 ¶ì¶ì¶ì¶î¶î¶î¶î¶ 2026-03-24 4/200 2026-03-24 16:41 by peike
[¿¼ÑÐ] 335Çóµ÷¼Á +4 yuyuÓî 2026-03-23 5/250 2026-03-23 23:49 by Txy@872106
[¿¼ÑÐ] Ò»Ö¾Ô¸¹ú¿Æ¹ý³ÌËù081700£¬274Çóµ÷¼Á +3 ÈýË®ÑÐ0Ë®Á¢·½ 2026-03-23 3/150 2026-03-23 23:11 by MajorWen
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û