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专业: 环境微生物学

[交流] 16S rDNA常用引物序列

16S rDNA常用引物序列
Normally, we used following primers to amplify bacterial 16S rRNA genes (27F and 1492R pair) and sequencing them (using other primers).
The primer sequencs are all listed in the reference: Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. Wiley, Chichester, pp 115-175
27F : 5’AGAGTTTGATCCTGGCTCAG 3' PCR and sequencing, most eubacteria
CTGAGCCAGGATCAAACTCT
357F : 5’ CTC CTA CGG GAG GCA GCA G 3' Most eubacteria
530F : 5’ GTG CCA GCA GCC GCG G 3' Most eubacteria and archaebacteria
926F : 5’ AAA CTC AAA GGA ATT GAC GG 3' Most eubacteria and archaebacteria
1114F : 5’ GCA ACG AGC GCA ACC C 3' Most eubacteria
342R : 5’ CTG CTG C CT CCC GTA G 3' Most eubacteria
519R : 5’ GTA TTA CCG CGG CTG CTG 3' Most eubacteria
anarchaebacteria
907R : 5’ CCG TCA ATT CCT TTG AGT TT 3' Most eubacteria and archaebacteria
1100R : 5’ GGG TTG CGC TCG TTG 3' Most eubacteria
1492R : 5’ TACGGCTACCTTGTTACGACTT 3' PCR and sequencing, most eubacteria
AAGTCGTAACAAGGTAGCCGTA
1525R : 5’ AAG GAG GTG ATC CAG CC 3' PCR and sequencing, most eubacteria

M=C:A, Y=C:T. K=G:T, R=A:G, S=G:C. W=A:T; all 1:1

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