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【答案】应助回帖
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感谢参与，应助指数 +1
jchen2014(sunshan4379代发): 金币+20 2014-11-26 09:18:21
sunshan4379: LS-EPI+1, 感谢应助！ 2014-11-26 09:18:28
Identification and distribution of four metabolites of geniposide in rats with adjuvant arthritis
作者:Chen, J (Chen, Jian)[ 1 ] ; Wu, H (Wu, Hong)[ 1 ] ; Dai, MM (Dai, Miao-Miao)[ 1 ] ; Li, H (Li, Hui)[ 1 ] ; Chen, JY (Chen, Jin-Yun)[ 1 ] ; Hu, SL (Hu, Shun-Li)[ 1 ]
FITOTERAPIA
卷: 97
页: 111-121
DOI: 10.1016/j.fitote.2014.05.023
出版年: SEP 2014
查看期刊信息
摘要
Geniposide (GE), also called Jasminoidin, is the major active ingredient of Gardenia jasminoides Ellis (GJ) fruit, which has long been used in traditional Chinese medicine (TCM). Growing evidences suggested that GE has a great potentiality for treating rheumatoid arthritis (RA). However, GE is rapidly metabolized, and we know little about its availability or metabolites in tissues. To elucidate the distribution of GE and its metabolites in tissues, three groups of adjuvant arthritis (AA) rats were given GE (33,66 and 120 mg/kg) from days 18 to 24, and the biotransformation of GE in plasma, liver, spleen, synovium, urine and mesenteric lymph node (MLN) of rats was investigated by a novel approach named Information-Dependent Acquisition (IDA)-Mediated LC-MS/MS method. As a result, GE and its four major metabolites were detected as follows: GE, G1, G2 in plasma; GE, G2 in MLNs; only GE in liver and synovium; GE, G2, G3 and G4 in spleen; and GE, Gl, G2 and G4 in urine. In total four metabolites (G1-G4) involved in the in vivo metabolism processes were identified. The results of this work have demonstrated the IDA-Mediated LC-MS/MS could screen rapidly and reliably the characterization of metabolites from iridoid compounds. (c) 2014 Elsevier B.V. All rights reserved.
关键词
作者关键词:Geniposide; Metabolites Information-Dependent Acquisition (IDA); LC-MS/MS; Adjuvant arthritis
KeyWords Plus:TANDEM MASS-SPECTROMETRY; INFORMATION-DEPENDENT ACQUISITION; RHEUMATOID-ARTHRITIS; GARDENIA-JASMINOIDES; SCREENING-PROCEDURE; GENIPIN; PHARMACOKINETICS; GLUCOSIDE; URINE; MS/MS
作者信息
通讯作者地址: Wu, H (通讯作者)
Anhui Univ Chinese Med, Coll Pharm, Hefei 230031, Peoples R China.
地址:
[ 1 ] Anhui Univ Chinese Med, Coll Pharm, Key Lab Modernized Chinese Med Anhui Prov, Hefei 230031, Anhui, Peoples R China
电子邮件地址:hongw@aliyun.com
基金资助致谢
基金资助机构 授权号
National Natural Science Foundation of China
81073122
Anhui Provincial Natural Science Research Project of University
KJ2009A045Z
youth Natural Science Foundation of Anhui University of Chinese Medicine
qn201308
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出版商
ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
类别 / 分类
研究方向 harmacology & Pharmacy
Web of Science 类别:Chemistry, Medicinal; Pharmacology & Pharmacy
文献信息
文献类型:Article
语种:English
入藏号: WOS:000341346400015
PubMed ID: 24910002
ISSN: 0367-326X
电子 ISSN: 1873-6971
期刊信息
Impact Factor (影响因子): Journal Citation Reports®
其他信息
IDS 号: AO4YD
Web of Science 核心合集中的 "引用的参考文献": 25
Web of Science 核心合集中的 "被引频次": 0 |
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