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【答案】应助回帖
★ ★ ★ ★ ★
感谢参与，应助指数 +1
eveb: 金币+5, ★★★★★最佳答案, thank 2014-11-13 12:27:03
jssxh: LS-EPI+1, 谢谢参与，请继续关注本版块 2014-11-13 14:59:39
Combined Antitumor Effect of Ad-bFGF-siRNA and Ad-Vpr on the Growth of Xenograft Glioma in Nude Mouse Model
作者:Zhang, B (Zhang, Biao)[ 2 ] ; Feng, XQ (Feng, Xuequan)[ 3 ] ; Wang, JH (Wang, Jinhuan)[ 1 ] ; Xu, XN (Xu, Xinnu)[ 4 ] ; Lin, N (Lin, Na)[ 4 ] ; Liu, HS (Liu, Hongsheng)[ 4 ]
PATHOLOGY & ONCOLOGY RESEARCH
卷: 17
期: 2
页: 237-242
DOI: 10.1007/s12253-010-9303-5
出版年: JUN 2011
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摘要
Basic fibroblast growth factor (bFGF) has been demonstrated to correlate with glioma grade and clinical outcome and has established its possible usefulness as a target for glioma therapy. Vpr has been described as an antitumor agent and displays a potent antitumor nature. Here, we try to investigate whether a combined treatment with bFGF-siRNA and Vpr gene would have a enhanced effectiveness on glioma in vitro and in vivo.After treatments with only Ad-bFGF-siRNA, only Ad-Vpr, and a combination of both, we assessed the changes in cell proliferation, cell cycle, and apoptosis in vitro by the methods of MTT, PI and FITC-AnnexinV double staining, respevtively. In addition, we also evaluated the combined effect of bFGF-siRNA and Vpr gene therapy on glioma in vivo using xenograft glioma models in nude mice. Combined Ad-bFGF-siRNA and Ad-Vpr treatment was more better successful in inhibiting cell proliferation in comparison with treatments of either Ad-bFGF-siRNA or Ad-Vpr alone. Treatment of Ad-Vpr alone or a treatment of a combination of Ad-bFGF-siRNA and Ad-Vpr induced the G2/M cell cycle arrest and apoptosis; however, combined treatment was more effective than the Ad-Vpr treatment alone. Although each single treatment can slow the growth of xenograft glioma, the combined treatment with Ad-bFGF-siRNA and Ad-Vpr was better than either the Ad-bFGF-siRNA or Ad-Vpr treatment alone. Our results suggest that the combination therapy with bFGF-siRNA and Vpr gene can achieve a enhanced activity of anti-glioma, supporting the idea that the combination of these two antitumor agents could open new perspectives in glioma therapy.
关键词
作者关键词:siRNA; bFGF; Vpr; Adenoviral vector
KeyWords Plus:IN-VIVO ELECTROPORATION; HIV-1 VPR; NUCLEAR ACCUMULATION; CELLS; GLIOBLASTOMA; INHIBITION; CASPASE-9; RECEPTOR; PLASMID; TUMORS
作者信息
通讯作者地址: Wang, JH (通讯作者)
Tianjin Huan Hu Hosp, Dept Neurosurg, 122 Qixiangtai Rd, Tianjin 300060, Peoples R China.
地址:
[ 1 ] Tianjin Huan Hu Hosp, Dept Neurosurg, Tianjin 300060, Peoples R China
[ 2 ] Tianjin Huan Hu Hosp, Clin Lab, Tianjin 300060, Peoples R China
[ 3 ] Tianjin First Ctr Hosp, Dept Neurosurg, Tianjin 300192, Peoples R China
[ 4 ] Tianjin First Ctr Hosp, Key Lab Crit Care Med, Minist Hlth, Tianjin 300192, Peoples R China
电子邮件地址:wangjinhuanfch@yahoo.com.cn
基金资助致谢
基金资助机构
授权号
National Natural Sciences Foundation of China
30672158
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出版商
SPRINGER, VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS
类别 / 分类
研究方向:Oncology; Pathology
Web of Science 类别:Oncology; Pathology
文献信息
文献类型:Article
语种:English
入藏号: WOS:000290587300007
PubMed ID: 20848251
ISSN: 1219-4956
期刊信息
Impact Factor (影响因子): Journal Citation Reports®
其他信息
IDS 号: 763VH
Web of Science 核心合集中的 "引用的参考文献": 20
Web of Science 核心合集中的 "被引频次": 5
引文网络
5 被引频次
20 引用的参考文献
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全部被引频次计数
5 / 所有数据库
5 / Web of Science 核心合集
1 / BIOSIS Citation Index
0 / 中国科学引文数据库
0 / Data Citation Index
0 / SciELO Citation Index
最近的引文
Zhang, S. HIV-1 viral protein R downregulates Ebp1 and stabilizes p53 in glioblastoma U87MG cells. CLINICAL & TRANSLATIONAL ONCOLOGY, MAR 2014.
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