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【答案】应助回帖
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感谢参与，应助指数 +1
eveb: 金币+5, ★★★★★最佳答案, thanks 2014-11-13 12:20:39
jssxh: LS-EPI+1, 谢谢参与，请继续关注本版块 2014-11-13 14:59:51
Adenovirus-mediated delivery of bFGF small interfering RNA increases levels of connexin 43 in the glioma cell line, U251
作者:Zhang, B (Zhang, Biao)[ 1 ] ; Feng, XQ (Feng, Xuequan)[ 2 ] ; Wang, JH (Wang, Jinhuan)[ 1,2 ] ; Xu, XN (Xu, Xinnu)[ 1 ] ; Liu, HS (Liu, Hongsheng)[ 1 ] ; Lin, N (Lin, Na)[ 1 ]
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
卷: 29
文献号: 3
DOI: 10.1186/1756-9966-29-3
出版年: JAN 14 2010
查看期刊信息
摘要
Background: bFGF is an important growth factor for glioma cell proliferation and invasion, while connexin 43 is implicated in the suppression of glioma growth. Correspondingly, gliomas have been shown to have reduced, or compromised, connexin 43 expression.
Methods: In this study, a bFGF-targeted siRNA was delivered to the glioma cell line, U251, using adenovirus (Ad-bFGF-siRNA) and the expression of connexin 43 and its phosphorylation state were evaluated. U251 cells were infected with Ad-bFGF-siRNA (100, 50, or 25 MOI), and infection with adenovirus expressing green fluorescent protein (Ad-GFP) at 100 MOI served as a control. Western blotting and immunofluorescence were used to detect the expression levels, phosphorylation, and localization of connexin 43 in U251 cells infected, and not infected, with Ad-bFGF-siRNA.
Results: Significantly higher levels of connexin 43 were detected in U251 cells infected with Ad-bFGF-siRNA at 100 and 50 MOI than in cells infected with Ad-GFP, and the same amount of connexin 43 was detected in Ad-GFP-infected and uninfected U251 cells. Connexin 43 phosphorylation did not differ between Ad-bFGF-siRNA-infected and uninfected U251 cells. However, the ratio of phosphorylated to unphosphorylated connexin 43 in Ad-bFGF-siRNA cells was lower, and connexin 43 was predominantly localized to the cytoplasm. Using a scrape loading dye transfer assay, more Lucifer Yellow was transferred to neighboring cells in the Ad-bFGF-siRNA treated group than in the control group.
Conclusion: To our knowledge, this is the first description of a role for connexin 43 in the inhibition of U251 growth using Ad-bFGF-siRNA.
关键词
KeyWords Plus:FIBROBLAST-GROWTH-FACTOR; HUMAN GLIOBLASTOMA CELLS; NUCLEAR ACCUMULATION; ASTROCYTIC TUMORS; DOWN-REGULATION; GAP-JUNCTIONS; EXPRESSION; CX43; INHIBITION; LOCALIZATION
作者信息
通讯作者地址: Wang, JH (通讯作者)
[显示增强组织信息的名称] Tianjin Med Univ, Key Lab Crit Care Med, Minist Hlth, Affiliated Tianjin Ctr Hosp 1, Tianjin 300192, Peoples R China.
地址:
[显示增强组织信息的名称] [ 1 ] Tianjin Med Univ, Key Lab Crit Care Med, Minist Hlth, Affiliated Tianjin Ctr Hosp 1, Tianjin 300192, Peoples R China
[显示增强组织信息的名称] [ 2 ] Tianjin Med Univ, Dept Neurosurg, Affiliated Tianjin Ctr Hosp 1, Tianjin 300192, Peoples R China
电子邮件地址:wangjinhuanfch@yahoo.com.cn
基金资助致谢
基金资助机构 授权号
National Natural Science Foundation of China
30672158
查看基金资助信息
出版商
BIOMED CENTRAL LTD, 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND
类别 / 分类
研究方向:Oncology
Web of Science 类别:Oncology
文献信息
文献类型:Article
语种:English
入藏号: WOS:000275445200001
PubMed ID: 20074329
ISSN: 1756-9966
期刊信息
Impact Factor (影响因子): Journal Citation Reports®
其他信息
IDS 号: 567KJ
Web of Science 核心合集中的 "引用的参考文献": 30
Web of Science 核心合集中的 "被引频次": 6 |
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