| 查看: 424 | 回复: 1 | ||
| 本帖产生 1 个 LS-EPI ，点击这里进行查看 | ||
eveb木虫 (正式写手)
|
[求助]
SCI收录及引用查询
|
|
|
作者: Biao, Zhang；Xuequan, Feng；Jinhuan, Wang；Xinnu, Xu；Hongsheng, Liu；Na, Lin 文题: Adenovirus-mediated delivery of bFGF small interfering RNA increases levels of connexin 43 in the glioma cell line, U251 期刊名,年份,卷(期),起止页码: Journal of experimental & clinical cancer research : 2010，29：3 全文链接: http://www.jeccr.com/content/29//3 数据库名称: pmc 收录信息: 求助 SCI 收录信息 被引次数 |
回复此楼» 猜你喜欢
参与限项
已经有3人回复
-
假如你的研究生提出不合理要求
已经有7人回复
-
实验室接单子
已经有4人回复
-
全日制（定向）博士
已经有4人回复
-
对氯苯硼酸纯化
已经有3人回复
-
求助:我三月中下旬出站，青基依托单位怎么办？
已经有12人回复
-
不自信的我
已经有12人回复
-
所感
已经有4人回复
-
要不要辞职读博？
已经有7人回复
-
北核录用
已经有3人回复
muse
捐助贵宾 (知名作家)
- LS-EPI: 147
- 应助: 314 (大学生)
- 贵宾: 0.549
- 金币: 20674
- 散金: 2783
- 红花: 81
- 沙发: 156
- 帖子: 6098
- 在线: 1468.2小时
- 虫号: 1207111
- 注册: 2011-02-19
- 专业: 保险学
【答案】应助回帖
★ ★ ★ ★ ★
感谢参与，应助指数 +1
eveb: 金币+5, ★★★★★最佳答案, thanks 2014-11-13 12:20:39
jssxh: LS-EPI+1, 谢谢参与，请继续关注本版块 2014-11-13 14:59:51
感谢参与，应助指数 +1
eveb: 金币+5, ★★★★★最佳答案, thanks 2014-11-13 12:20:39
jssxh: LS-EPI+1, 谢谢参与，请继续关注本版块 2014-11-13 14:59:51
|
Adenovirus-mediated delivery of bFGF small interfering RNA increases levels of connexin 43 in the glioma cell line, U251 作者:Zhang, B (Zhang, Biao)[ 1 ] ; Feng, XQ (Feng, Xuequan)[ 2 ] ; Wang, JH (Wang, Jinhuan)[ 1,2 ] ; Xu, XN (Xu, Xinnu)[ 1 ] ; Liu, HS (Liu, Hongsheng)[ 1 ] ; Lin, N (Lin, Na)[ 1 ] JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH 卷: 29 文献号: 3 DOI: 10.1186/1756-9966-29-3 出版年: JAN 14 2010 查看期刊信息 摘要 Background: bFGF is an important growth factor for glioma cell proliferation and invasion, while connexin 43 is implicated in the suppression of glioma growth. Correspondingly, gliomas have been shown to have reduced, or compromised, connexin 43 expression. Methods: In this study, a bFGF-targeted siRNA was delivered to the glioma cell line, U251, using adenovirus (Ad-bFGF-siRNA) and the expression of connexin 43 and its phosphorylation state were evaluated. U251 cells were infected with Ad-bFGF-siRNA (100, 50, or 25 MOI), and infection with adenovirus expressing green fluorescent protein (Ad-GFP) at 100 MOI served as a control. Western blotting and immunofluorescence were used to detect the expression levels, phosphorylation, and localization of connexin 43 in U251 cells infected, and not infected, with Ad-bFGF-siRNA. Results: Significantly higher levels of connexin 43 were detected in U251 cells infected with Ad-bFGF-siRNA at 100 and 50 MOI than in cells infected with Ad-GFP, and the same amount of connexin 43 was detected in Ad-GFP-infected and uninfected U251 cells. Connexin 43 phosphorylation did not differ between Ad-bFGF-siRNA-infected and uninfected U251 cells. However, the ratio of phosphorylated to unphosphorylated connexin 43 in Ad-bFGF-siRNA cells was lower, and connexin 43 was predominantly localized to the cytoplasm. Using a scrape loading dye transfer assay, more Lucifer Yellow was transferred to neighboring cells in the Ad-bFGF-siRNA treated group than in the control group. Conclusion: To our knowledge, this is the first description of a role for connexin 43 in the inhibition of U251 growth using Ad-bFGF-siRNA. 关键词 KeyWords Plus:FIBROBLAST-GROWTH-FACTOR; HUMAN GLIOBLASTOMA CELLS; NUCLEAR ACCUMULATION; ASTROCYTIC TUMORS; DOWN-REGULATION; GAP-JUNCTIONS; EXPRESSION; CX43; INHIBITION; LOCALIZATION 作者信息 通讯作者地址: Wang, JH (通讯作者) [显示增强组织信息的名称] Tianjin Med Univ, Key Lab Crit Care Med, Minist Hlth, Affiliated Tianjin Ctr Hosp 1, Tianjin 300192, Peoples R China. 地址: [显示增强组织信息的名称] [ 1 ] Tianjin Med Univ, Key Lab Crit Care Med, Minist Hlth, Affiliated Tianjin Ctr Hosp 1, Tianjin 300192, Peoples R China [显示增强组织信息的名称] [ 2 ] Tianjin Med Univ, Dept Neurosurg, Affiliated Tianjin Ctr Hosp 1, Tianjin 300192, Peoples R China 电子邮件地址:wangjinhuanfch@yahoo.com.cn 基金资助致谢 基金资助机构 授权号 National Natural Science Foundation of China 30672158 查看基金资助信息 出版商 BIOMED CENTRAL LTD, 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND 类别 / 分类 研究方向:Oncology Web of Science 类别:Oncology 文献信息 文献类型:Article 语种:English 入藏号: WOS:000275445200001 PubMed ID: 20074329 ISSN: 1756-9966 期刊信息 Impact Factor (影响因子): Journal Citation Reports® 其他信息 IDS 号: 567KJ Web of Science 核心合集中的 "引用的参考文献": 30 Web of Science 核心合集中的 "被引频次": 6 |
2楼2014-11-13 12:11:24