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| Ultrasensitive immunoassay for free prostate specific antigen based on ferrocenecarboxylate enhanced cathodic electrochemiluminescence of peroxydisulfate. Microchimica Acta, 2014, 181:1285–1291. |
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muse
捐助贵宾 (知名作家)
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【答案】应助回帖
★ ★ ★ ★ ★
感谢参与，应助指数 +1
jssxh: 金币+5, LS-EPI+1, 谢谢参与，请继续关注本版块。 2014-10-24 14:37:15
感谢参与，应助指数 +1
jssxh: 金币+5, LS-EPI+1, 谢谢参与，请继续关注本版块。 2014-10-24 14:37:15
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Ultrasensitive immunoassay for free prostate-specific antigen based on ferrocenecarboxylate enhanced cathodic electrochemiluminescence of peroxydisulfate 作者:Zhang, F (Zhang, Fei)[ 1 ] ; Mao, L (Mao, Li)[ 1 ] ; Zhu, MQ (Zhu, Meiqiong)[ 1 ] MICROCHIMICA ACTA 卷: 181 期: 11-12 页: 1285-1291 DOI: 10.1007/s00604-014-1240-7 出版年: AUG 2014 查看期刊信息 摘要 The catalytic effect of ferrocenecarboxylate (FCC; in the form of nanocrystals) on the cathodic electrochemiluminescence (ECL) of peroxodisulfate was studied and applied to an ECL immunoassay to quantify free prostate-specific antigen (fPSA). The nanocrystals were entrapped in graphene previously functionalized with poly(dimethyldiallylammonium chloride) to give a solid-state nanohybrid referred to as FCC-Gr-PDDA. When cast onto a glassy carbon electrode (GCE), the ECL of peroxodisulfate is strongly enhanced. An immunoelectrode was then constructed by coating the positively charged nanohybrids matrix of FCC-Gr-PDDA with negatively charged gold nanoparticles and then immobilizing antibody against fPSA on the Au-NPs. The resulting electrode is then incubated with samples containing fPSA. An immunocomplex is formed in the presence of fPSA, and this prevents the solid-state nanohybrid film to enhance the ECL of the peroxodisulfate luminophor. The observed decrease in ECL intensity is directly related to the concentration of fPSA antigen in the range from 0.005 to 5 ng mL(-1), and the detection limit is as low as 1.7 pg mL(-1). The method is deemed to provide a widely applicable and general platform for ECL-based immunoassays. 关键词 作者关键词:Electrochemiluminescence; Immunoassay; Ferrocenecarboxylate; Peroxydisulfate; Free prostate-specific antigen KeyWords Plus:ELECTROGENERATED CHEMILUMINESCENCE; QUANTUM DOTS; BIOSENSOR; SYSTEM; AMPLIFICATION; NANOPARTICLES; ELECTRODES; KINETICS; CANCER; FILM 作者信息 通讯作者地址: Mao, L (通讯作者) China West Normal Univ, Coll Chem & Chem Engn, Chem Synth & Pollut Control Key Lab Sichuan Prov, Nanchong 637000, Peoples R China. 地址: [ 1 ] China West Normal Univ, Coll Chem & Chem Engn, Chem Synth & Pollut Control Key Lab Sichuan Prov, Nanchong 637000, Peoples R China 电子邮件地址:limaochem@outlook.com 基金资助致谢 基金资助机构 授权号 National Natural Science Foundation (NNSF) of China 21277109 Applied Basic Research Program of Sichuan Provincial Science and Technology Department 2011JYZ020 Youth Science Foundation of Sichuan Province education department 122B146 program for scientific and technological innovative Team in Sichuan Province universities 2010008 Startup Project for Doctor scientific research in CWNU 1113003 Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province 12CSPC(1-7) 查看基金资助信息 出版商 SPRINGER WIEN, SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA 类别 / 分类 研究方向:Chemistry Web of Science 类别:Chemistry, Analytical 文献信息 文献类型:Article 语种:English 入藏号: WOS:000340360900014 ISSN: 0026-3672 电子 ISSN: 1436-5073 期刊信息 Impact Factor (影响因子): Journal Citation Reports® 其他信息 IDS 号: AN1RI Web of Science 核心合集中的 "引用的参考文献": 32 Web of Science 核心合集中的 "被引频次": 0 |
2楼2014-10-24 12:52:33
muse
捐助贵宾 (知名作家)
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3楼2014-10-24 12:52:45