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maoligl

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Ultrasensitive immunoassay for free prostate specific antigen based on ferrocenecarboxylate enhanced cathodic electrochemiluminescence of peroxydisulfate. Microchimica Acta, 2014, 181:1285–1291.

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jssxh: 金币+5, LS-EPI+1, 谢谢参与，请继续关注本版块。 2014-10-24 14:37:15

Ultrasensitive immunoassay for free prostate-specific antigen based on ferrocenecarboxylate enhanced cathodic electrochemiluminescence of peroxydisulfate

作者:Zhang, F (Zhang, Fei)[ 1 ] ; Mao, L (Mao, Li)[ 1 ] ; Zhu, MQ (Zhu, Meiqiong)[ 1 ]

MICROCHIMICA ACTA

卷: 181

期: 11-12

页: 1285-1291

DOI: 10.1007/s00604-014-1240-7

出版年: AUG 2014

查看期刊信息
摘要

The catalytic effect of ferrocenecarboxylate (FCC; in the form of nanocrystals) on the cathodic electrochemiluminescence (ECL) of peroxodisulfate was studied and applied to an ECL immunoassay to quantify free prostate-specific antigen (fPSA). The nanocrystals were entrapped in graphene previously functionalized with poly(dimethyldiallylammonium chloride) to give a solid-state nanohybrid referred to as FCC-Gr-PDDA. When cast onto a glassy carbon electrode (GCE), the ECL of peroxodisulfate is strongly enhanced. An immunoelectrode was then constructed by coating the positively charged nanohybrids matrix of FCC-Gr-PDDA with negatively charged gold nanoparticles and then immobilizing antibody against fPSA on the Au-NPs. The resulting electrode is then incubated with samples containing fPSA. An immunocomplex is formed in the presence of fPSA, and this prevents the solid-state nanohybrid film to enhance the ECL of the peroxodisulfate luminophor. The observed decrease in ECL intensity is directly related to the concentration of fPSA antigen in the range from 0.005 to 5 ng mL(-1), and the detection limit is as low as 1.7 pg mL(-1). The method is deemed to provide a widely applicable and general platform for ECL-based immunoassays.
关键词

作者关键词:Electrochemiluminescence; Immunoassay; Ferrocenecarboxylate; Peroxydisulfate; Free prostate-specific antigen

KeyWords Plus:ELECTROGENERATED CHEMILUMINESCENCE; QUANTUM DOTS; BIOSENSOR; SYSTEM; AMPLIFICATION; NANOPARTICLES; ELECTRODES; KINETICS; CANCER; FILM
作者信息

通讯作者地址: Mao, L (通讯作者)
China West Normal Univ, Coll Chem & Chem Engn, Chem Synth & Pollut Control Key Lab Sichuan Prov, Nanchong 637000, Peoples R China.

地址:
[ 1 ] China West Normal Univ, Coll Chem & Chem Engn, Chem Synth & Pollut Control Key Lab Sichuan Prov, Nanchong 637000, Peoples R China

电子邮件地址:limaochem@outlook.com
基金资助致谢
基金资助机构授权号
National Natural Science Foundation (NNSF) of China
21277109
Applied Basic Research Program of Sichuan Provincial Science and Technology Department
2011JYZ020
Youth Science Foundation of Sichuan Province education department
122B146
program for scientific and technological innovative Team in Sichuan Province universities
2010008
Startup Project for Doctor scientific research in CWNU
1113003
Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province
12CSPC(1-7)
查看基金资助信息
出版商

SPRINGER WIEN, SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA
类别 / 分类

研究方向:Chemistry

Web of Science 类别:Chemistry, Analytical
文献信息

文献类型:Article

语种:English

入藏号: WOS:000340360900014

ISSN: 0026-3672

电子 ISSN: 1436-5073
期刊信息

Impact Factor (影响因子): Journal Citation Reports®

其他信息

IDS 号: AN1RI

Web of Science 核心合集中的 "引用的参考文献": 32

Web of Science 核心合集中的 "被引频次": 0