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DH-PS induced the secretions of multiple cytokines and chemokines in vivo In order to understand the immunological activities of DH-PS, we investigated the profiles of cytokines and chemokines induced by DH-PS in mice. BALB/c mice (n= 3 for DH-PS and phosphate buffered saline (PBS) group) were injected intraperitoneally (IP) with DH-PS (300 mg/mouse) in PBS or PBS only and the sera were collected at 0 (before injection), 2 and 18 hrs after the injection of DH-PS for the detection of cytokines and chemokines, which were quantified by Beadlyte Mouse 21-plex Cytokine Detection system and ELISA kits (for IL-1ra). Compared with PBS group shown in Figure 1, the increase of TNF-a, IL-12 p40, IL-6, IL-10 and RANTES were observed at 2 hrs, along with robust inductions of KC, MCP-1 and MIP-1b. An apparent increase in IL-1b at 2 hrs was noted but it did not reach statistical significance. These results suggested that DH-PS induced Th1 (IL-12 p40), Th2 (IL-6, IL-10), inflammatory cytokines (TNF-a) and chemokines (KC, RANTES, MCP-1, MIP-1b), which might further modulate the downstream activations of immune cells. Furthermore, an antiinflammatory molecule, IL-1ra, was increased from 465 to 4199 pg/ml (,9 folds of PBS control group) at 2 hrs. This indicated that the amounts of IL-1ra induced were 10 folds more than IL-1b (increased from 144 to 397 pg/ml, ,1.6 folds of PBS control group), suggesting that DH-PS-induced IL-1ra might override the activity induced by IL-1b.  1.jpg |
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hym0411
木虫 (正式写手)
- 翻译EPI: 1
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RXMCDM: 金币+1, 多谢应助！ 2014-09-19 23:20:43
AnnF: 金币+20, 翻译EPI+1, ★★★★★最佳答案, 谢谢，迟来的奖励 2014-09-21 11:51:09
RXMCDM: 金币+1, 多谢应助！ 2014-09-19 23:20:43
AnnF: 金币+20, 翻译EPI+1, ★★★★★最佳答案, 谢谢，迟来的奖励 2014-09-21 11:51:09
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自己翻译的，纰漏众多，敬请指正，相互交流，共同进步，望采纳。 关于DH-PS在体内诱导多种细胞因子和趋化因子的分泌的研究 为了阐明DH-PS的免疫学活性，我们研究了DH-PS在小鼠体内诱导的细胞因子和趋化因子的特性。BALB/c小鼠（DH-PS磷酸盐缓冲液组，3只）用DH-PS磷酸盐缓冲液（300mg/只）腹腔注射，磷酸盐缓冲液单独注射做对照，并在注射前，注射后2小时、18小时分别取血清，用于检测细胞因子和趋化因子。定量方法为Beadlyte Mouse 21-plex Cytokine Detection system（仪器）和酶联免疫试剂盒。试剂组和对照组的比较见图一，在2小时时，TNF-α，IL-12p40,IL-6,IL-10和RANTES都有增加，而且能够强烈的诱导产生KC，MCP-1和MIP-1等趋化因子。2小时时IL-1b的增加很明显，但并没有达到统计学意义上的显著性差异。这些表明DH-PS能够诱导产生Th1（IL-12p40）,Th2(IL-6,IL-10)，炎症性细胞因子（TNF-α）和趋化因子（KC，RANTES，MCP-1，MIP-1b）等，而这些因子可能会进一步调节其下游免疫细胞的活性。而且，抗炎分子IL-1ra在2小时的血清含量从465pg/ml增加到4199pg/ml（是对照组的9倍）。这表明诱导产生的IL-1ra的量可能会是IL-1b的10倍之多（IL-1b从144pg/ml增加到397pg/ml，是对照组的1.6倍），预示着DH-ps诱导产生的IL-1ra可能会掩盖IL-1b诱导产生的活性。 |
2楼2014-09-19 23:00:16
