DH-PS induced the secretions of multiple cytokines and
chemokines in vivo
In order to understand the immunological activities of DH-PS,
we investigated the profiles of cytokines and chemokines induced
by DH-PS in mice. BALB/c mice (n= 3 for DH-PS and phosphate
buffered saline (PBS) group) were injected intraperitoneally (IP)
with DH-PS (300 mg/mouse) in PBS or PBS only and the sera
were collected at 0 (before injection), 2 and 18 hrs after the
injection of DH-PS for the detection of cytokines and chemokines,
which were quantified by Beadlyte Mouse 21-plex Cytokine
Detection system and ELISA kits (for IL-1ra). Compared with PBS
group shown in Figure 1, the increase of TNF-a, IL-12 p40, IL-6,
IL-10 and RANTES were observed at 2 hrs, along with robust
inductions of KC, MCP-1 and MIP-1b. An apparent increase in
IL-1b at 2 hrs was noted but it did not reach statistical significance.
These results suggested that DH-PS induced Th1 (IL-12 p40), Th2
(IL-6, IL-10), inflammatory cytokines (TNF-a) and chemokines
(KC, RANTES, MCP-1, MIP-1b), which might further modulate
the downstream activations of immune cells. Furthermore, an antiinflammatory
molecule, IL-1ra, was increased from 465 to
4199 pg/ml (,9 folds of PBS control group) at 2 hrs. This
indicated that the amounts of IL-1ra induced were 10 folds more
than IL-1b (increased from 144 to 397 pg/ml, ,1.6 folds of PBS
control group), suggesting that DH-PS-induced IL-1ra might override
the activity induced by IL-1b.
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