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1.Here, we use a primer set designed to detect the full taxonomic diversity of fungal small subunit ribosomal RNAgenes 2.Group-specifc environmental PCR followed by cloning and gene library construction provides a means of comparing subsets of microbial diversity between environ-ments, detecting low-abundance microbes and improvings ampling effciency |
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zchui112
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1.Here, we use a primer set designed to detect the full taxonomic diversity of fungal small subunit ribosomal RNAgenes ÕâÀï,ÎÒÃÇʹÓÃÒ»Ì×Éè¼ÆºÃµÄÒýÎïÈ¥¼ì²âÕæ¾úºËÌÇÌåСÑÇ»ùRNA»ùÒòµÄ·ÖÀàѧµÄ¶àÑùÐÔ. 2.Group-specifc environmental PCR followed by cloning and gene library construction provides a means of comparing subsets of microbial diversity between environ-ments, detecting low-abundance microbes and improvings ampling effciency ÒÀ¿¿¿Ë¡µÄÌØÒìÐÔ×éȺ»·¾³PCR¼¼Êõ(˵ʵÔÚ»°,Õâ¸öºÜ±ðŤ,µ«ÊÇÕâÖÖPCRÎÒȷʵ´ÓδÌý¹ý)ºÍ»ùÒòÎÄ¿âµÄ½¨Á¢Îª»·¾³Î¢ÉúÎï×Ó¼¯¶àÑùÐԵıȽÏ,µÍ·á¶È¶È΢ÉúÎïµÄ¼ì²âºÍÀ©ÔöЧÂʵÄÌá¸ßÌṩÁË·½·¨. |
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