In order to trace back the origin of 18:3, the major lipid classes of plastids, MGDG, DGDG, and phosphatidylglycerol (PG), as well as those of the ER, phosphytidylcholine (PC), phosphatidylethanolamine (PE), and phosphatidylinositol (PI), were analyzed. the total amounts of MGDG (21.76-fold,P= 0.022), DGDG (22.36-fold,P=0.032),andPG(21.25-fold,P=0.153) were decreased in crown gall tumors, and again, the ER-specific phospholipid pools, PC (1.79-fold, 0.05), PE (1.31-fold, P= 0.29), and PI (2.61-fold,P= 0.002), were increased (Fig. 1B). Within all classes of the ER-derived lipids, the levels of those containing 18:3 were significantly (P#0.05) higher in tumors compared with reference stems (Fig. 1, C¨CH).
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