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Colorimetric detection of mercury, lead and copper ions simultaneously using protein-functionalized gold nanoparticles ×÷Õß:Guo, YM (Guo, Yongming)[ 1,2,3 ] ; Wang, Z (Wang, Zhuo)[ 2 ] ; Qu, WS (Qu, Weisi)[ 2 ] ; Shao, HW (Shao, Huawu)[ 1 ] ; Jiang, XY (Jiang, Xingyu)[ 2 ] BIOSENSORS & BIOELECTRONICS ¾í: 26 ÆÚ: 10 Ò³: 4064-4069 DOI: 10.1016/j.bios.2011.03.033 ³ö°æÄê: JUN 15 2011 ²é¿´ÆÚ¿¯ÐÅÏ¢ ÕªÒª A simple, cost-effective and rapid colorimetric method for any or all of Hg2+ Pb2+ and Cu2+ detection using papain-functionalized gold nanoparticles (P-AuNPs) has been developed. Papain is a protein with seven cystein residues, which can selectively bind with Hg2+, Pb2+ and Cu2+. We functionalized gold nanoparticles with papain. The P-AuNPs could be used to simultaneously detect Hg2+, Pb2+ and Cu2+, and showed different responses to the three ions in an aqueous solution based on the aggregation-induced color change of gold nanoparticles. The P-AuNPs displayed the most obvious response to mercury ions in water in contrast to lead and copper ions, and the real water sample analysis verified the conclusion. The sensitivity of the detection system was influenced by the pH of the P-AuNPs solution, the concentration of P-AuNPs and the size of gold nanoparticles, and we found that larger gold nanoparticles contributed to more sensitive results. The detection system can detect as low as 200 nM Hg2+, Pb2+ or Cu2+ using 42 nm gold nanoparticles. We expect our approach to have wide-ranging applications in the developing region for monitoring water quality in some areas. (C) 2011 Elsevier B.V. All rights reserved. ¹Ø¼ü´Ê ×÷Õ߹ؼü´Ê:Gold nanoparticles; Mercury ions; Lead ions; Copper ions; Protein; Colorimetric detection KeyWords Plus IRECTED SYNTHESIS; METAL-IONS; OLIGONUCLEOTIDES; CHEMISTRY; PAPAIN; ASSAY×÷ÕßÐÅÏ¢ ͨѶ×÷ÕßµØÖ·: Shao, HW (ͨѶ×÷Õß) ÏÔʾÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ Chinese Acad Sci, Chengdu Inst Biol, Nat Prod Res Ctr, Chengdu 610041, Sichuan, Peoples R China. µØÖ·: ÏÔʾÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ [ 1 ] Chinese Acad Sci, Chengdu Inst Biol, Nat Prod Res Ctr, Chengdu 610041, Sichuan, Peoples R China ÏÔʾÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ [ 2 ] Natl Ctr Nanosci & Technol, Beijing 100190, Peoples R China ÏÔʾÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ [ 3 ] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China µç×ÓÓʼþµØÖ·:wangz@nanoctr.cn; shaohw@cib.ac.cn; xingyujiang@nanoctr.cn ²é¿´×÷Õßʶ±ðºÅ×÷Õßʶ±ðºÅ: »ù½ð×ÊÖúÖÂл »ù½ð×ÊÖú»ú¹¹ ÊÚȨºÅ National Natural Science Foundation of China 90813032 20890020 21025520 Chinese Academy of Sciences KJCX2-YW-M15 Scientific Research Foundation for the Returned Overseas Chinese Scholars, the Ministry of Science and Technology 2009CB30001 2011CB933201 Ministry of Human Resources and Social Security of the People's Republic of China ²é¿´»ù½ð×ÊÖúÐÅÏ¢ ³ö°æÉÌ ELSEVIER ADVANCED TECHNOLOGY, OXFORD FULFILLMENT CENTRE THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND Àà±ð / ·ÖÀà Ñо¿·½Ïò:Biophysics; Biotechnology & Applied Microbiology; Chemistry; Electrochemistry; Science & Technology - Other Topics Web of Science Àà±ð:Biophysics; Biotechnology & Applied Microbiology; Chemistry, Analytical; Electrochemistry; Nanoscience & Nanotechnology ÎÄÏ×ÐÅÏ¢ ÎÄÏ×ÀàÐÍ:Article ÓïÖÖ:English Èë²ØºÅ: WOS:000291904000014 ISSN: 0956-5663 ÆäËûÐÅÏ¢ IDS ºÅ: 781AW Web of Science ºËÐĺϼ¯ÖÐµÄ "ÒýÓõIJο¼ÎÄÏ×": 33 Web of Science ºËÐĺϼ¯ÖÐµÄ "±»ÒýƵ´Î": 46 |

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