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【答案】应助回帖
★ ★ ★ ★ ★ 感谢参与,应助指数 +1 2008guoym: 金币+5 2014-06-07 18:54:12 oven1986: 检索EPI+1, 感谢应助! 2014-06-07 23:41:47
Colorimetric detection of mercury, lead and copper ions simultaneously using protein-functionalized gold nanoparticles
作者:Guo, YM (Guo, Yongming)[ 1,2,3 ] ; Wang, Z (Wang, Zhuo)[ 2 ] ; Qu, WS (Qu, Weisi)[ 2 ] ; Shao, HW (Shao, Huawu)[ 1 ] ; Jiang, XY (Jiang, Xingyu)[ 2 ]
BIOSENSORS & BIOELECTRONICS
卷: 26 期: 10 页: 4064-4069
DOI: 10.1016/j.bios.2011.03.033
出版年: JUN 15 2011
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摘要
A simple, cost-effective and rapid colorimetric method for any or all of Hg2+ Pb2+ and Cu2+ detection using papain-functionalized gold nanoparticles (P-AuNPs) has been developed. Papain is a protein with seven cystein residues, which can selectively bind with Hg2+, Pb2+ and Cu2+. We functionalized gold nanoparticles with papain. The P-AuNPs could be used to simultaneously detect Hg2+, Pb2+ and Cu2+, and showed different responses to the three ions in an aqueous solution based on the aggregation-induced color change of gold nanoparticles. The P-AuNPs displayed the most obvious response to mercury ions in water in contrast to lead and copper ions, and the real water sample analysis verified the conclusion. The sensitivity of the detection system was influenced by the pH of the P-AuNPs solution, the concentration of P-AuNPs and the size of gold nanoparticles, and we found that larger gold nanoparticles contributed to more sensitive results. The detection system can detect as low as 200 nM Hg2+, Pb2+ or Cu2+ using 42 nm gold nanoparticles. We expect our approach to have wide-ranging applications in the developing region for monitoring water quality in some areas. (C) 2011 Elsevier B.V. All rights reserved.
关键词
作者关键词:Gold nanoparticles; Mercury ions; Lead ions; Copper ions; Protein; Colorimetric detection
KeyWords Plus IRECTED SYNTHESIS; METAL-IONS; OLIGONUCLEOTIDES; CHEMISTRY; PAPAIN; ASSAY
作者信息
通讯作者地址: Shao, HW (通讯作者)
显示增强组织信息的名称 Chinese Acad Sci, Chengdu Inst Biol, Nat Prod Res Ctr, Chengdu 610041, Sichuan, Peoples R China.
地址:
显示增强组织信息的名称 [ 1 ] Chinese Acad Sci, Chengdu Inst Biol, Nat Prod Res Ctr, Chengdu 610041, Sichuan, Peoples R China
显示增强组织信息的名称 [ 2 ] Natl Ctr Nanosci & Technol, Beijing 100190, Peoples R China
显示增强组织信息的名称 [ 3 ] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
电子邮件地址:wangz@nanoctr.cn; shaohw@cib.ac.cn; xingyujiang@nanoctr.cn
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基金资助致谢
基金资助机构 授权号
National Natural Science Foundation of China
90813032
20890020
21025520
Chinese Academy of Sciences
KJCX2-YW-M15
Scientific Research Foundation for the Returned Overseas Chinese Scholars, the Ministry of Science and Technology
2009CB30001
2011CB933201
Ministry of Human Resources and Social Security of the People's Republic of China
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出版商
ELSEVIER ADVANCED TECHNOLOGY, OXFORD FULFILLMENT CENTRE THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND
类别 / 分类
研究方向:Biophysics; Biotechnology & Applied Microbiology; Chemistry; Electrochemistry; Science & Technology - Other Topics
Web of Science 类别:Biophysics; Biotechnology & Applied Microbiology; Chemistry, Analytical; Electrochemistry; Nanoscience & Nanotechnology
文献信息
文献类型:Article
语种:English
入藏号: WOS:000291904000014
ISSN: 0956-5663
其他信息
IDS 号: 781AW
Web of Science 核心合集中的 "引用的参考文献": 33
Web of Science 核心合集中的 "被引频次": 46 |
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