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新虫 (小有名气)

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虫号: 1650696
注册: 2012-02-28
专业: 环境污染化学

[求助] 土壤粒径分离

大家好，我看了一些英文文献，可不是很明白他们到底是使用的什么仪器做到土壤粒径分离的，In triplicates, the fine earth was separated into 5 particle-size
fractions using a two-step sample dispersion for minimization
of artefacts (Amelung and Zech, 1999). First, ultrasonic
dispersion with an energy input of 60 J ml−1 and a pressureabove 5 g (Amelung and Zech, 1999) was carried out on 30 g
amounts of soil in 150 ml of deionized water. The energy input
of the sonicator (Branson W 450) was calibrated according
to North (1976). After separation of the coarse sand fraction
(250–2000 μm) by wet-sieving, the remaining suspension
(300 ml) was treated ultrasonically (440 J ml−1) at a soil to
water ratio of 1:10 (v/v) for additional dispersion of more stable
microaggregates. The fine sand (63–250 μm) was isolated by
wet-sieving. The silt (2–63 μm) and clay (b2 μm) fractions
were gained by centrifugation of the remaining suspension at
40 ×g. This allowed the silt to be settled while the clay fraction
stayed suspended and was decanted into a suitable container.
The sediment was resuspended in 300 ml of water and
centrifuged again. This procedure was repeated at least 15
times until the supernatant remained clear after centrifugation.
All fractions were dried at 40 °C.希望有做这方面的朋友能告知谢谢