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´ó¼ÒºÃ£¬ÎÒ¿´ÁËһЩӢÎÄÎÄÏ×£¬¿É²»ÊǺÜÃ÷°×ËûÃǵ½µ×ÊÇʹÓõÄʲôÒÇÆ÷×öµ½ÍÁÈÀÁ£¾¶·ÖÀëµÄ£¬In triplicates, the fine earth was separated into 5 particle-size
fractions using a two-step sample dispersion for minimization
of artefacts (Amelung and Zech, 1999). First, ultrasonic
dispersion with an energy input of 60 J ml−1 and a pressureabove 5 g (Amelung and Zech, 1999) was carried out on 30 g
amounts of soil in 150 ml of deionized water. The energy input
of the sonicator (Branson W 450) was calibrated according
to North (1976). After separation of the coarse sand fraction
(250¨C2000 ¦Ìm) by wet-sieving, the remaining suspension
(300 ml) was treated ultrasonically (440 J ml−1) at a soil to
water ratio of 1:10 (v/v) for additional dispersion of more stable
microaggregates. The fine sand (63¨C250 ¦Ìm) was isolated by
wet-sieving. The silt (2¨C63 ¦Ìm) and clay (b2 ¦Ìm) fractions
were gained by centrifugation of the remaining suspension at
40 ¡Ág. This allowed the silt to be settled while the clay fraction
stayed suspended and was decanted into a suitable container.
The sediment was resuspended in 300 ml of water and
centrifuged again. This procedure was repeated at least 15
times until the supernatant remained clear after centrifugation.
All fractions were dried at 40 ¡ãC.Ï£ÍûÓÐ×öÕâ·½ÃæµÄÅóÓÑÄܸæÖªÐ»Ð»
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