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It is important and useful for highly effective, economical and safe use of herbicides to screen and breed of herbicide-tolerant rice and find effective additives. Our recent results indicated that japonica rice show higher sensitivity to bispyribac-sodium compared to indica rice. Futher analysis found that there are differences between indica and japonica rice, such as the activity of glutathione S-transferase (GST), contents of cytochrome P450 and cytochrome b5, relative gene expression of GSTU9 and CYP72A23 after treated with bispyribac-sodium. Thus, the different amounts of GSH, GSSG or CytP450 between indica and japonica rice were determined after treated with bispyribac-sodium. the differences of GST and CytP450 were measured between indica and japonica rice, such as the activities of GST and CytP450,the gene sequence, gene expression and protein level of GSTU9 and CYP72A23, in order to elucidate the role of GST and CytP450 in different sensitivities of indica and japonica rice. Combining with related mutants, the function of GSTU9 and CYP72A23 in different sensitivities of indica and japonica rice would be further investigated in this project.The activity inducers or inhibitors were screened for effective use of bispyribac-sodium The results would be contributed to explore the metabolism and increase the application of bispyribac-sodium.

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wc4646: ½ð±Ò+30, ·­ÒëEPI+1, ¡ï¡ï¡ïºÜÓаïÖú, ·Ç³£¸Ðл¥Ö÷°ï档лл¡£ 2014-03-14 10:37:59
The screening  and breeding of herbicide-tolerant rice and the finding of  effective additives is of importance to highly effective, economical and safe use of herbicides  . Our recent results found that japonica rice show higher sensitivity to bispyribac-sodium than  indica rice. Futher analysis  indicated that it is due to theer  , such as the activity of glutathione S-transferase (GST), contents of cytochrome P450 and cytochrome b5, related gene expression of GSTU9 and CYP72A23 after treatment of bispyribac-sodium.Thus, the different amounts of GSH, GSSG or CytP450 of indica and japonica rice were determined after treatment of   bispyribac-sodium. The differences of GST and CytP450 between indica and japonica rice were measured , such as the activities of GST and CytP450, gene sequence, gene expression and protein level of GSTU9 and CYP72A23, in order to elucidate the role of GST and CytP450 in the indica and japonica rice different sensitivities of bispyribac-sodium.Through analysis of  related mutants, the function of GSTU9 and CYP72A23 in different sensitivities of indica and japonica rice will  be further investigated in this project.The activity inducers or inhibitors were screened for the effective use of bispyribac-sodium .These  findings will not only reveal  the  scientific significance of the mechanism of indica and japonica GST and CytP450 different Bispyribac sensitivity  , but also  provide a scientific basis for the efficient and safe use of Bispyribac in  rice fields.
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