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wc4646木虫 (正式写手)
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[求助]
求助如下英语能否予以修改润色，谢谢
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| It is important and useful for highly effective, economical and safe use of herbicides to screen and breed of herbicide-tolerant rice and find effective additives. Our recent results indicated that japonica rice show higher sensitivity to bispyribac-sodium compared to indica rice. Futher analysis found that there are differences between indica and japonica rice, such as the activity of glutathione S-transferase (GST), contents of cytochrome P450 and cytochrome b5, relative gene expression of GSTU9 and CYP72A23 after treated with bispyribac-sodium. Thus, the different amounts of GSH, GSSG or CytP450 between indica and japonica rice were determined after treated with bispyribac-sodium. the differences of GST and CytP450 were measured between indica and japonica rice, such as the activities of GST and CytP450,the gene sequence, gene expression and protein level of GSTU9 and CYP72A23, in order to elucidate the role of GST and CytP450 in different sensitivities of indica and japonica rice. Combining with related mutants, the function of GSTU9 and CYP72A23 in different sensitivities of indica and japonica rice would be further investigated in this project.The activity inducers or inhibitors were screened for effective use of bispyribac-sodium The results would be contributed to explore the metabolism and increase the application of bispyribac-sodium. |
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wc4646
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培育筛选耐除草剂的水稻材料以及发现有效助剂，是除草剂高效、经济和安全使用的重要保证。我们前期工作发现，籼稻和粳稻对除草剂双草醚的敏感性不一；进一步分析发现，籼稻和粳稻的谷胱甘肽硫转移酶（GST）活性和细胞色素P450氧化酶系（CytP450）含量不一。本项目拟以粳稻日本腈和籼稻9311为试材，比较双草醚胁迫下GST反应底物（GSH、GSSG）和CytP450含量差异时序变化；通过体内和体外测定，比较应答对双草醚的酶活特性；比较GSTU9和CYP72A23基因和其编码产物的结构差异和应答双草醚的表达特征；通过遗传突变体，揭示GSTU9和CYP72A23在耐受双草醚中的贡献；添加酶活诱导剂或抑制剂，分析双草醚进一步高效应用的有效助剂。以上研究结果，不仅对于揭示籼稻与粳稻中GST和CytP450对双草醚敏感性差异的机理具有科学意义，而且为水稻田中高效安全应用双草醚提供科学依据。 |
3楼2014-03-12 08:30:14
RXMCDM
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2楼2014-03-12 02:32:57
RXMCDM
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【答案】应助回帖
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wc4646: 金币+30, 翻译EPI+1, ★★★很有帮助, 非常感谢楼主帮忙。谢谢。 2014-03-14 10:37:59
wc4646: 金币+30, 翻译EPI+1, ★★★很有帮助, 非常感谢楼主帮忙。谢谢。 2014-03-14 10:37:59
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The screening and breeding of herbicide-tolerant rice and the finding of effective additives is of importance to highly effective, economical and safe use of herbicides . Our recent results found that japonica rice show higher sensitivity to bispyribac-sodium than indica rice. Futher analysis indicated that it is due to theer , such as the activity of glutathione S-transferase (GST), contents of cytochrome P450 and cytochrome b5, related gene expression of GSTU9 and CYP72A23 after treatment of bispyribac-sodium.Thus, the different amounts of GSH, GSSG or CytP450 of indica and japonica rice were determined after treatment of bispyribac-sodium. The differences of GST and CytP450 between indica and japonica rice were measured , such as the activities of GST and CytP450, gene sequence, gene expression and protein level of GSTU9 and CYP72A23, in order to elucidate the role of GST and CytP450 in the indica and japonica rice different sensitivities of bispyribac-sodium.Through analysis of related mutants, the function of GSTU9 and CYP72A23 in different sensitivities of indica and japonica rice will be further investigated in this project.The activity inducers or inhibitors were screened for the effective use of bispyribac-sodium .These findings will not only reveal the scientific significance of the mechanism of indica and japonica GST and CytP450 different Bispyribac sensitivity , but also provide a scientific basis for the efficient and safe use of Bispyribac in rice fields. 大致修改如下，看看行不？ |
4楼2014-03-14 09:39:18
