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The pre-fixed small vessels were pre-embedded into 1.5% agar, and its position and lumen were adjusted. Using self-made agar embedding mould could be convenient obtaining the agar block with complete structure and right size. Then did the paraffin embedding in accordance with the normal procedure of dehydration and embedding. Finally made paraffin sections. The results indicated that using the double embedding method had kept the integrity of lumen, the intima and other tissues of vessel hadn¡¯t lost during the whole embedding procedure, and the ideal light microscope slices were finally harvested.
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¿ÆÑÐÈ˲Å: ½ð±Ò+200, ¡ï¡ï¡ïºÜÓаïÖú, Ê®·Ö¸Ðл£¬ ·îÉϽð±Ò200 2014-03-08 16:34:07
The fixed small vessels was pre-embedded into 1.5% agar in advance, and its position and lumen were adjusted well. By using self-made agar embedding mould,  the agar block in complete structure and right size  can be obtained conveniently.  Then paraffin embedding was did according to the conventional dehydration and embedding procedure, and staining was the last step. The results indicated that,  by using the double embedding method, the integrity of lumen is kept, the intima and other tissues of vessel are not damaged and lost during the whole embedding procedure, and the ideal light microscope slices were got also.
Hallelujah
2Â¥2014-03-06 22:31:57
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