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since_90

至尊木虫 (知名作家)

[求助] 跪求大神支招,Genbank这么说怎么解释 已有2人参与

While processing your GenBank submissions KF933350-KF933352, we have
come across an issue that requires your attention.

Internal stop codons have been detected in the conceptual translation
of the following coding regions:

KF933351
KF933352

If the nucleotide sequences are incorrect, send us the corrected
sequences as a FASTA set and we will replace the current sequences
in your submission.

However, if the sequences in your submission are correct, please
explain the presence of these internal stop codons.

If you are not able to resolve the internal stop codons, your
records can be processed. However, the following note will be
added in the COMMENT field of those with stop codons:

COMMENT GenBank staff is unable to verify sequence and/or annotation
provided by the submitter.

In addition, the relevant information will be removed and the files
will indicate that the data are unverified. Sequences flagged as
unverified will not be included in NCBI BLAST databases.



就是简单的2个16S-rDNA的序列,根本不知道怎么解释为什么会有内终止子存在,咋办
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Valar morghulis.
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wulv008

银虫 (正式写手)

你好,你的这个问题是怎么解决的?我也遇见这样的问题了,谢谢
我相信,坚持+努力=成功!
7楼2014-03-14 16:41:17
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ifyousee

新虫 (小有名气)

【答案】应助回帖

★ ★
感谢参与,应助指数 +1
gyesang: 金币+2, 鼓励回帖交流! 2014-02-26 17:58:16
Internal stop codons have been detected in the conceptual translation
of the following coding regions:
鬼子们序列感觉序列里面有终止密码子,觉得事情变得非常奇怪了。于是让你:如果觉得序列是正确的给他个解释;或是序列错误了给他发个正确版本的序列
2楼2014-02-26 11:27:49
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since_90

至尊木虫 (知名作家)



问题是不知道为啥会有终止密码子的存在,咋回复能糊弄过去
Valar morghulis.
3楼2014-02-26 16:40:42
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gyesang

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优秀版主优秀版主

【答案】应助回帖

感谢参与,应助指数 +1
引用回帖:
3楼: Originally posted by since_90 at 2014-02-26 16:40:42


问题是不知道为啥会有终止密码子的存在,咋回复能糊弄过去

你想糊弄?
人家已经给你几个选择了:
1. 提供正确的序列
2. 不能提供正确的序列,你给个解释,为什么会有internal stop codons
3. 你不能提供正确序列,也不能解释,人家也会给你处理序列,就是加上那么一句话。

其实,你可以再搞一次测序,确认一下你的序列,如果的确是你提交那样的,那没办法,只能提交,如果的确有误,就改正过来再提交。
学术问题讨论咨询发邮件gyesang@163.com
4楼2014-02-26 18:10:49
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