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1¡¢Even if a lot of different aptamers sequences have been tested, there is no information on the dilution correction or optical density correction for fluorescence intensity. 2¡¢It would be good to know, even if the concentration of thrombin added (fig2) is low (1nM), that the dilution correction have been done (page 18, in detection of protein) 3¡¢I know by experience that it is a structure very difficult to obtain and that has to be verified. But maybe the fact that the porphyrin is linking and fluoresce is enough£¿ ʲôÊÇ dilution correction £¿ÔõôÀí½âÕâ¸ö¸ÅÄ µÚÈý¸öÎÊÌâתÕÛºóÃæÊÇÏë±í´ïÊ²Ã´ÄØ£¿ Çë¸ßÊÖÃÇÖ¸µã¡£ |
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