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守望天空8633

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[资源] Chem. Soc. Rev. 最新综述：交替的激光激发---单分子FRET（荧光共振能量转移）

最新一期的Chemical Society Review, 发表了荷兰瓦赫宁根大学的Johannes Hohlbein关于交替激光激发和荧光共振能量转移的综述。
交替激光激发扩展了荧光基对生物体系的整体评价。交替激光激发可以对的荧光标记物的种类和数目进行分类筛选，从而使得综合运用交替激光激发和单分子荧光共振能量转移获得成功。交替激光激发为确定准确的分子间距离提供了方便的方法。本文对交替激光激发的概念和当前应用进行了综述，并重点讨论了如何通过FRET的范围获得矫正的距离。同时为交替激光激发对推动单分子荧光共振能量转移实验在解决复杂生物体系时空分辨的研究提供了新想法。
Abstract: The alternating-laser excitation (ALEX) scheme continues to expand the possibilities of fluorescence-based assays to study biological entities and interactions. Especially the combination of ALEX and single-molecule Förster Resonance Energy Transfer (smFRET) has been very successful as ALEX enables the sorting of fluorescently labelled species based on the number and type of fluorophores present. ALEX also provides a convenient way of accessing the correction factors necessary for determining accurate molecular distances. Here, we provide a comprehensive overview of the concept and current applications of ALEX and we explicitly discuss how to obtain fully corrected distance information across the entire FRET range. We also present new ideas for applications of ALEX which will push the limits of smFRET-based experiments in terms of temporal and spatial resolution for the study of complex biological systems.

截图01_副本.jpg[ Last edited by 守望天空8633 on 2013-9-17 at 19:17 ]

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附件 1 : Alternating-laserexcitationsingle-moleculeFRETandbeyond(CSR,20).pdf

2013-09-17 19:17:00, 3.88 M