±±¾©Ê¯ÓÍ»¯¹¤Ñ§Ôº2026ÄêÑо¿ÉúÕÐÉú½ÓÊÕµ÷¼Á¹«¸æ
²é¿´: 1235  |  »Ø¸´: 5

pushizi

ľ³æ (СÓÐÃûÆø)

pushizi

[ÇóÖú] ʾ×ÙÉ«ËØ¸²¸ÇÔÚµ°°×Ìõ´øÉÏ-western blot½á¹ûºÜÄÑ¿´-Çó½â¾ö

×î½üÔÚ½øÐÐÒ»¸öԼΪ14 kD´óСµ°°×µÄwestern blotʵÑ飬ºÜÓôÃÆµÄÊÇ£ºµçÓ¾³öÀ´ºó£¬Îҵĵ°°×Ìõ´ø¾ÍÔÚloading bufferµÄָʾ¼Á£¨¿¼Âí˹ÁÁÀ¶£¨G250£©£©µÄÀ¶É«²¿·ÖÇøÓò£¬½«Æäתµ½ÏõËáÏËÎ¬ËØÄ¤ºó£¬À¶É«µÄȾÁÏÒ²ÄÜתµ½Ä¤ÉÏ£¬ÏÔÉ«ÍêÌõ´ø¾Í¸úÀ¶É«µÄ½»ÔÓÔÚÒ»Æð£¬·Ç³£ÄÑ¿´¡£Ë¼¿¼ÒÔϽâ¾ö·½°¸£¬Çë´ó¼Ò¶àÌὨÒ飬ÄÄÌõ¿ÉÐУº
£¨1£©»»ÓýºµÄŨ¶È£ºÏÖÔÚÓõÄÊÇ15%µÄ½º¡£ÊÇ·ñÓ¦¸Ã»»ÓÃ20%µÄ½ºÅ¨¶È£¿¿´Ò»Ð©Ìû×ÓÀïºÃÏñָʾ¼ÁµÄ¿ìÂýÔÚ²»Í¬µÄ½ºÅ¨¶ÈÖв»Í¬¡£20%µÄ½ºÖÐָʾ¼ÁµÄ´óСÔÚÄÄÀÓÐûÓгæÓÑ×ö¹ý°¡£¿ÁíÍ⣬14kDµÄµ°°×ÊDz»ÊÇÓÃ20%µÄ½ºÅ¨¶ÈÓеã¿äÕŰ¡£¿
£¨2£©½«Ê¾×Ù¼Á»»Îªäå·ÓÀ¶£¿¿´Ò»Ð©Ìû×ÓÖÐÓÐÈË˵äå·ÓÀ¶ÄÜÅÜÔÚ7 kDµÄµ°°×ÒÔÏ£¬ÓÖÓÐÈË˵ÔÚ17kD×óÓÒ£¬ÓзñÈ·¶¨µÄ˵·¨°¡£¿ÇëעϽºÅ¨¶È~~
£¨3£©ÄÜ·ñ²»¼Óָʾ¼Á°¡£¿½öÓøÊÓͳÁµíµ°°×£¬È»ºóÓÃԤȾmarker½øÐÐָʾ£¿Õâ¸ö¹ý³ÌÓÐÈË×ö¹ýô£¿»áÓÐÎÊÌâô£¿ÁíÍ⣬¸ÊÓ͵ÄÖÕŨ¶È²ÉÓöàÉٱȽϺ𡣿
×îºó£¬ÔÙÇë½Ì´ó¼ÒÒ»¸öÎÊÌ⣬ÔÚ×öwestern blotµÄ¹ý³ÌÖУ¬Ìõ´ø×ÜÊÇÒç³ö£¬ÎÒ²»È·¶¨Ã¿¸öÑùÆ·£¨È«Ï¸°ûÁѽâÒº£©ÖÐÎÒµÄÄ¿µÄµ°°×ÓжàÉÙ£¬ÄÇôÎÒ³ýÁË·´¸´ÊÔÑ飬¼õÉÙÉÏÑùÁ¿Ö®Í⣬ÓÐûÓÐÆäËûʲô¿ìËÙ¼ò±ãµÄ·½·¨À´½â¾ö£¿
¸Ðл´ÓÍ·¿´µ½Î²µÄÅóÓÑÃÇ£¡¸ü¼Ó¸ÐлÄܹ»ÎªÎÒ½â´ðµÄ¸÷λºÃÐÄÈË£¡ÔÚ´ËÏÈл¹ý£¡£¡
»Ø¸´´ËÂ¥
µ«Ô¸¿ìÀÖ²»Í£Ðª£¬±¯É˲»ÔÙÀ´~
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

yimingwang

½ð³æ (СÓÐÃûÆø)

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï
¸Ðл²ÎÓ룬ӦÖúÖ¸Êý +1
pushizi: ½ð±Ò+10, ¡ï¡ï¡ïºÜÓаïÖú 2013-08-10 09:59:13
kx444555: ½ð±Ò+1, ¹ÄÀø½»Á÷ 2013-08-10 12:00:00
15%µÄ½ºÃ»ÓÐÎÊÌâ°¡£¬Ò»°ãµÄloading bufferÔÚ10k×óÓÒ¡£14kÍêÈ«ÄÜ·Ö¿ª¡£×ªÄ¤Ç°ÓÃTowbin buffer¶àÏ´Ò»»á¶ù£¬loading buffer»áµôµÄ¡£ÔÙת¾ÍûɶÎÊÌâÁË¡£Western×ã¹»ÁéÃô£¬ÄãûÓбØÒªÉÏÌ«¶àÑùµÄ¡£¼õÉÙÉÏÑùÁ¿¾ÍÐÐÁË¡£
Let'sdoit.
2Â¥2013-08-10 00:25:48
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

pushizi

ľ³æ (СÓÐÃûÆø)

pushizi

ÒýÓûØÌû:
2Â¥: Originally posted by yimingwang at 2013-08-10 00:25:48
15%µÄ½ºÃ»ÓÐÎÊÌâ°¡£¬Ò»°ãµÄloading bufferÔÚ10k×óÓÒ¡£14kÍêÈ«ÄÜ·Ö¿ª¡£×ªÄ¤Ç°ÓÃTowbin buffer¶àÏ´Ò»»á¶ù£¬loading buffer»áµôµÄ¡£ÔÙת¾ÍûɶÎÊÌâÁË¡£Western×ã¹»ÁéÃô£¬ÄãûÓбØÒªÉÏÌ«¶àÑùµÄ¡£¼õÉÙÉÏÑùÁ¿¾ÍÐÐÁË¡£

ÇëÎÊÄúµÄloading bufferÀïÃæµÄȾÁÏÊÇʲô°¡£¿
µ«Ô¸¿ìÀÖ²»Í£Ðª£¬±¯É˲»ÔÙÀ´~
3Â¥2013-08-10 10:00:27
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

yimingwang

½ð³æ (СÓÐÃûÆø)

¡¾´ð°¸¡¿Ó¦Öú»ØÌû

¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï
pushizi: ½ð±Ò+20, ¡ï¡ï¡ïºÜÓаïÖú 2013-08-13 13:26:45
ÒýÓûØÌû:
3Â¥: Originally posted by pushizi at 2013-08-10 03:00:27
ÇëÎÊÄúµÄloading bufferÀïÃæµÄȾÁÏÊÇʲô°¡£¿...

4x sample buffer 10 mL :  
2.0 ml 1M Tris-HCl pH 6.8
    0.8 g SDS
    4.0 ml 100% glycerol
    0.4 ml 14.7 M ¦Â-mercaptoethanol
    1.0 ml 0.5 M EDTA
    8 mg bromophenol Blue
Let'sdoit.
4Â¥2013-08-13 03:46:55
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

pushizi

ľ³æ (СÓÐÃûÆø)

pushizi

ÒýÓûØÌû:
4Â¥: Originally posted by yimingwang at 2013-08-13 03:46:55
4x sample buffer 10 mL :  
2.0 ml 1M Tris-HCl pH 6.8
    0.8 g SDS
    4.0 ml 100% glycerol
    0.4 ml 14.7 M ¦Â-mercaptoethanol
    1.0 ml 0.5 M EDTA
    8 mg bromophenol Blue...

ºÃµÄ£¡·Ç³£¸Ðл£¡
ºóÀ´ÓÖ×ÉѯÁËÆäËûÈË£¬È·ÊµÓ¦¸ÃÓÃäå·ÓÀ¶Ã»ÓÐÎÊÌâ¡£
ÓõÚÈýÖÖ½â¾ö·½·¨Ò²ÄÜÓã¬Ó¦¸Ã¸ü±£ÏÕ~
µ«Ô¸¿ìÀÖ²»Í£Ðª£¬±¯É˲»ÔÙÀ´~
5Â¥2013-08-13 13:28:03
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

Allen206

Òø³æ (СÓÐÃûÆø)

°ÑÐÞ·ÒÀ¼È¥µô²»¾ÃÐÐÁË£¬¸ù¾Ý¾­Ñ鶨ʱ¼ä£¬±ð³öÈ¥¾ÍºÃÀ²£¬
²»Ïë˵ʲô£¬£¬£¬£¬
6Â¥2013-08-13 17:47:05
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
Ïà¹Ø°æ¿éÌø×ª ÎÒÒª¶©ÔÄÂ¥Ö÷ pushizi µÄÖ÷Ìâ¸üÐÂ
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] ÉúÎïѧѧ˶Çóµ÷¼Á£º351·ÖÒ»Ö¾Ô¸ÄϾ©Ê¦·¶´óѧÉúÎïѧרҵ +4 ¡­¡«¡¢Íõ¡­¡« 2026-04-06 5/250 2026-04-06 10:45 by ´óÁ¦Ë®ÊÖÁ¦´óÎÞÇ
[¿¼ÑÐ] 0857´óÀà»·¾³¹¤³ÌBÇøÇóµ÷¼Á +3 ¹¨ÓíÃú 2026-04-05 3/150 2026-04-06 10:22 by À¶ÔÆË¼Óê
[¿¼ÑÐ] 302·Ö 085601Çóµ÷¼ÁÍÆ¼ö +11 zyxÉϰ¶£¡ 2026-04-05 11/550 2026-04-05 22:13 by dongzh2009
[¿¼ÑÐ] ÉúÎ﹤³ÌÇóµ÷¼Á +6 ϲ»¶»¹ÊDz»¸ÊÐÄ 2026-04-05 6/300 2026-04-05 10:28 by ÌÆãå¶ù
[¿¼ÑÐ] ²ÄÁϹ¤³Ì085601Êý¶þÓ¢Ò»335Çóµ÷¼Á +6 Ë«ÂíβƦÀϰå2 2026-03-31 6/300 2026-04-04 22:29 by hemengdong
[¿¼ÑÐ] ²ÄÁϵ÷¼Á +15 Ò»ÑùYWY 2026-04-01 15/750 2026-04-04 22:23 by hemengdong
[¿¼ÑÐ] 272Çóµ÷¼Á +4 Ëɰس£Çà5 2026-04-03 4/200 2026-04-04 17:03 by babysonlkd
[¿¼ÑÐ] ÍÁľ304Çóµ÷¼Á +4 ÍÃͻͻͻ£¬ 2026-03-31 4/200 2026-04-04 13:34 by 1753564080
[¿¼ÑÐ] ¹¤¿Æ341·Öµ÷¼Á +3 Âå¶àÂÞ 2026-04-03 3/150 2026-04-03 14:20 by 1753564080
[¿¼ÑÐ] ÖØÇì´óѧ²ÄÁÏÓ뻯¹¤085600£¬³õÊÔ370+£¬ÇóÇóµ÷¼Á½¨Òé +8 shzhou_ 2026-04-01 9/450 2026-04-03 09:31 by À¶ÔÆË¼Óê
[¿¼ÑÐ] Ò»Ö¾Ô¸ÉÂÎ÷ʦ·¶´óѧÉúÎïѧ317·Ö +5 1563ÈÕ¡£ 2026-04-02 5/250 2026-04-03 06:58 by ilovexiaobin
[¿¼ÑÐ] ½»Í¨ÔËÊ俼ÊÔ264·ÖÇ󹤿Ƶ÷¼Á +4 jike777 2026-04-02 4/200 2026-04-02 21:53 by zllcz
[¿¼ÑÐ] 348Çóµ÷¼Á +6 ÎâÑå׿24k 2026-04-02 6/300 2026-04-02 14:07 by ¸øÄãÄã×¢ÒâÐÝÏ¢
[¿¼ÑÐ] Ò»Ö¾Ô¸±±¾©¿Æ¼¼£¬085601×Ü·Ö305Çóµ÷¼Á +9 °ëÉú¹Ï£¡ 2026-04-01 11/550 2026-04-02 08:28 by Wang200018
[¿¼ÑÐ] 0817»¯¹¤Ñ§Ë¶µ÷¼Á +11 ŬÁ¦Éϰ¶ÖУ¡ 2026-03-31 11/550 2026-04-01 20:30 by Àµ´ºÑÞ
[¿¼ÑÐ] 318Çóµ÷¼Á +8 ÆßÒä77 2026-04-01 8/400 2026-04-01 10:37 by Jaylen.
[¿¼ÑÐ] µ÷¼ÁÉêÇë +8 ÕÅÕÅÕÅÕÅzy 2026-03-31 9/450 2026-04-01 08:29 by zjbkx
[¿¼ÑÐ] 254²ÄÁÏÓ뻯¹¤Çóµ÷¼Á +3 º²¶¬ÁÖéª 2026-03-30 4/200 2026-03-31 17:53 by yishunmin
[¿¼ÑÐ] 323·Ö ʳƷÓëÓªÑøµ÷¼Á +3 ºÙooo 2026-03-31 3/150 2026-03-31 09:38 by longlotian
[¿¼ÑÐ] 293Çóµ÷¼Á +3 ĩδmm 2026-03-30 5/250 2026-03-30 17:23 by Íõ±£½Ü33
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û