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小木虫论坛-学术科研互动平台 » 生物医药区 » 分子生物 » 综合其他 » 请问有没有研究活性氧与抗生素作用的，求助。。。。
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韩江钓雪

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[交流] 请问有没有研究活性氧与抗生素作用的，求助。。。。

科研想做一下关于活性氧与抗生素是否有相关作用的研究。
但不知道用活性氧是用什么好呢？有这方面经验的虫友麻烦来两句。
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1楼 2013-07-12 11:01:03
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韩江钓雪

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myprayer: 金币+1, 哈哈，楼主可真是厉害呀。 2013-07-13 18:51:55
下午把science上最近的3篇关于抗生素与ros关系的文章下载了。摘要如下：
1.Killing by bactericidal antibiotics does not depend on reactive oxygen species.

Abstract
Bactericidal antibiotics kill by modulating their respective targets. This traditional view has been challenged by studies that propose an alternative, unified mechanism of killing, whereby toxic reactive oxygen species (ROS) are produced in the presence of antibiotics. We found no correlation between an individual cell's probability of survival in the presence of antibiotic and its level of ROS. An ROS quencher, thiourea, protected cells from antibiotics present at low concentrations, but the effect was observed under anaerobic conditions as well. There was essentially no difference in survival of bacteria treated with various antibiotics under aerobic or anaerobic conditions. This suggests that ROS do not play a role in killing of bacterial pathogens by antibiotics.
2 Cell death from antibiotics without the involvement of reactive oxygen species.
Abstract
Recent observations have suggested that classic antibiotics kill bacteria by stimulating the formation of reactive oxygen species (ROS). If true, this notion might guide new strategies to improve antibiotic efficacy. In this study, the model was directly tested. Contrary to the hypothesis, antibiotic treatment did not accelerate the formation of hydrogen peroxide in Escherichia coli and did not elevate intracellular free iron, an essential reactant for the production of lethal damage. Lethality persisted in the absence of oxygen, and DNA repair mutants were not hypersensitive, undermining the idea that toxicity arose from oxidative DNA lesions. We conclude that these antibiotic exposures did not produce ROS and that lethality more likely resulted from the direct inhibition of cell-wall assembly, protein synthesis, and DNA replication.
3.
Fe-S cluster biosynthesis controls uptake of aminoglycosides in a ROS-less death pathway.
Abstract
All bactericidal antibiotics were recently proposed to kill by inducing reactive oxygen species (ROS) production, causing destabilization of iron-sulfur (Fe-S) clusters and generating Fenton chemistry. We find that the ROS response is dispensable upon treatment with bactericidal antibiotics. Furthermore, we demonstrate that Fe-S clusters are required for killing only by aminoglycosides. In contrast to cells, using the major Fe-S cluster biosynthesis machinery, ISC, cells using the alternative machinery, SUF, cannot efficiently mature respiratory complexes I and II, resulting in impendence of the proton motive force (PMF), which is required for bactericidal aminoglycoside uptake. Similarly, during iron limitation, cells become intrinsically resistant to aminoglycosides by switching from ISC to SUF and down-regulating both respiratory complexes. We conclude that Fe-S proteins promote aminoglycoside killing by enabling their uptake
但这几篇文章都是说抗生素抗菌机制跟细菌内ros产生没有关系。而我的想法是ros是否能协助抗生素抗菌，这样应该还能做吧。求指导。。。。。@凌波丽 @ixido @Haibara_Ai
顺便把附件贴上供大家交流讨论。
http://pan.baidu.com/share/link? ... p;amp;uk=4095909806
http://pan.baidu.com/share/link? ... p;amp;uk=4095909806
http://pan.baidu.com/share/link? ... p;amp;uk=4095909806
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12楼2013-07-13 17:29:05
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凌波丽

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小木虫: 金币+0.5, 给个红包，谢谢回帖
kx444555: 金币+1, 鼓励交流 2013-07-12 11:52:22
活性氧与抗生素是否有相关作用用FTIR很容易检测出来，你选如何展开讨论。
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2楼2013-07-12 11:37:44
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韩江钓雪

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2楼: Originally posted by 凌波丽 at 2013-07-12 11:37:44
活性氧与抗生素是否有相关作用用FTIR很容易检测出来，你选如何展开讨论。

我想要让二者加在细菌上看抑菌效果和单用抗生素的区别。这样怎们做？活性氧有很多种，用哪种好呢？
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3楼2013-07-12 11:48:08
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凌波丽

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小木虫: 金币+0.5, 给个红包，谢谢回帖
kx444555: 金币+2, 鼓励交流 2013-07-12 13:26:27
韩江钓雪: 金币+5, 谢谢亲 2013-07-13 10:41:56
引用回帖:
3楼: Originally posted by 韩江钓雪 at 2013-07-12 11:48:08
我想要让二者加在细菌上看抑菌效果和单用抗生素的区别。这样怎们做？活性氧有很多种，用哪种好呢？...

把两者同时加到细菌的培养基里就行，培养皿要密闭（加盖），测定不同浓度的对比的抗生素和活性氧的量，感测细菌生长情况；同时在细胞外用FTIR光谱测定同样的浓度的对比的抗生素和活性氧彼此混合与分离条件下的光谱变化。
活性氧有很多种，用哪种好呢？我不清楚，你多试几种吧，但是要注意活性氧本身就会抑制细菌生长和杀菌，所以每次二者加在细菌上看抑菌效果不仅要有单用抗生素，还要有单用活性氧的抑菌效果的对比。
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4楼2013-07-12 11:55:12
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