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牛皮糖1025

金虫 (小有名气)

[求助] 分析了蛋白质信号肽,但是不太会看记过,怎么看有没有信号肽?三条线分别代表什么?

英语不太好,实验室也没人做这一块,哎,各位大侠帮帮忙吧
分析了蛋白质信号肽,但是不太会看记过,怎么看有没有信号肽?三条线分别代表什么?
信号肽预测.png
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牛皮糖1025

金虫 (小有名气)

引用回帖:
4楼: Originally posted by mitocam at 2013-05-24 11:09:04
C-score (raw cleavage site score)
    The output from the CS networks, which are trained to distinguish signal peptide cleavage sites from everything else.
    Note the position numbering of the cl ...

我看过这个,不太明白呵呵
5楼2013-05-24 11:47:25
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lxj341401

至尊木虫 (著名写手)

【答案】应助回帖


感谢参与,应助指数 +1
牛皮糖1025(gyesang代发): 金币+1, 鼓励回帖应助! 2013-05-24 03:05:55
有没有信号肽不用看这张图,在图的下方会写signal是YES OR NO,如果是NO,就是没有信号肽,如果是YES,再看YES 前面的位置,会标出在哪两个氨基酸之间。
2楼2013-05-23 15:16:28
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牛皮糖1025

金虫 (小有名气)

引用回帖:
2楼: Originally posted by lxj341401 at 2013-05-23 15:16:28
有没有信号肽不用看这张图,在图的下方会写signal是YES OR NO,如果是NO,就是没有信号肽,如果是YES,再看YES 前面的位置,会标出在哪两个氨基酸之间。

那这三个曲线分别代表什么?
3楼2013-05-24 09:44:30
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mitocam

新虫 (正式写手)

【答案】应助回帖

感谢参与,应助指数 +1
C-score (raw cleavage site score)
    The output from the CS networks, which are trained to distinguish signal peptide cleavage sites from everything else.
    Note the position numbering of the cleavage site: the C-score is trained to be high at the position immediately after the cleavage site (the first residue in the mature protein).
S-score (signal peptide score)
    The output from the SP networks, which are trained to distinguish positions within signal peptides from positions in the mature part of the proteins and from proteins without signal peptides.
Y-score (combined cleavage site score)
    A combination (geometric average) of the C-score and the slope of the S-score, resulting in a better cleavage site prediction than the raw C-score alone. This is due to the fact that multiple high-peaking C-scores can be found in one sequence, where only one is the true cleavage site. The Y-score distinguishes between C-score peaks by choosing the one where the slope of the S-score is steep.
4楼2013-05-24 11:09:04
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