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An ideal cellular probe for practical application should minimally perturb living systems at the concentrations employed. Accordingly, the cytotoxicities of solvent INR1 and INR2 were determined using the MTT assay. In general, at a compound concentration of 5 μM, cellular viability was estimated as greater than 90% after incubation for 2 h (Fig. 10), and higher concentration (10μM) can result in decreased cell survival, 76% for INR1 and 85% for INR2. The results indicate that the probes generally present low toxicity for living cell imaging under the conditions applied.
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