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¡°The tubes were then autoclaved at 115¡æ for 10 min and final readings were taken following autoclaving and additional 24 h incubation at room temperature. ¡° Çó½ÌÕâ¾ä»°ÈçºÎÀí½â£¿ÔÎÄÈçÏ¡£ Electrolyte leakage (EL) was measured by using a conductivity meter. Samples were cut into equal sized pieces (0.3 g per treatment) and placed in 25 mm ¡Á150 mm culture vessels containing 15 ml of distilled water, and shaken at 100 rpm on an orbital shaker for 24 h at room temperature. The initial conductance of the bathing solution was measured using a conductivity meter. The tubes were then autoclaved at 115¡æ for 10 min and final readings were taken following autoclaving and additional 24 h incubation at room temperature. EL (%) was calculated as initial measurements/final measurements¡Á 100. ÎҵķÒëÈçÏ£º²ÉÓõ絼ÒǶԵç½âÖÊÉøÍ¸ÂʽøÐвâÁ¿¡£½«ÑùÆ·¼ô³ÉÏàͬ´óСµÄС¿é£¨Ã¿´¦Àí0.3¿Ë£©£¬·ÅÈë25 mm ¡Á150 mmÅàÑøÆ¿ÖУ¨15mlÕôÁóË®£©£¬ÔÚÊÒÎÂÌõ¼þÏÂÓÚÕñµ´Æ÷ÖÐÒÔ100 rpmµÄËÙ¶ÈÕðµ´24Сʱ¡£Óõ絼ÒDzâÁ¿µç½âÒºµÄ³õʼµçµ¼ÂÊ¡£½«ÊÔ¹ÜÔÚ115¡æÏ¸ßѹÕôÆû´¦Àí10min£¬24СʱºòÔÙ²âÁ¿µçµ¼ÂÊ¡£EL (%)Öµ=¼ÆËã³õʼ²âÁ¿Öµ/×îÖÕ²âÁ¿Öµ¡Á 100¡£ |
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hookhans
Ìú¸Ëľ³æ (ÖøÃûдÊÖ)
Farmer
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- Ó¦Öú: 186 (¸ßÖÐÉú)
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- ×¢²á: 2013-01-25
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