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Electrolyte leakage (EL) was measured by using a conductivity meter. Samples were cut into equal sized pieces (0.3 g per treatment) and placed in 25 mm ¡Á150 mm culture vessels containing 15 ml of distilled water, and shaken at 100 rpm on an orbital shaker for 24 h at room temperature. The initial conductance of the bathing solution was measured using a conductivity meter. The tubes were then autoclaved at 115¡æ for 10 min and final readings were taken following autoclaving and additional 24 h incubation at room temperature. EL (%) was calculated as initial measurements/final measurements¡Á 100.
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The tubes were then autoclaved at 115¡æ for 10 min and final readings were taken following autoclaving and additional 24 h incubation at room temperature.
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