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15Â¥: Originally posted by ÀËÂþµÄÂí at 2013-03-29 19:42:13
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PMSF may be one kind of protease inhibitors. I often use a protease inbibitor cocktail, it is a mixture of several kinds of inhibitors. I have no idea about the protein you work with now, so you may have to check some literatures about this protein: extration, WB protocol and so on. you can check it online, google or NCBI, etc. you shoul be taught to do this by your supervisor, not me.
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14Â¥: Originally posted by ÀËÂþµÄÂí at 2013-03-29 19:26:39
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OK, protease inhbitor may be one of the causes, not the main one. You still have to check a lot of key points. we bought it from a company, but I forgot its name. I can give you my protocl if you are interested in, just send me a Email: dengqiong11@mails.jlu.edu.cn
17Â¥2013-03-30 00:34:34
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