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¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¸Ðл²ÎÓ룬ӦÖúÖ¸Êý +1 jingyu501: ½ð±Ò+20, ¡ï¡ï¡ï¡ï¡ï×î¼Ñ´ð°¸, thanks 2013-03-09 16:57:38
Rosmarinic Acid from Eelgrass Shows Nematicidal and Antibacterial Activities against Pine Wood Nematode and Its Carrying Bacteria
×÷Õß: Wang, JY (Wang, Jingyu)[ 1 ] ; Pan, XR (Pan, Xueru)[ 1 ] ; Han, Y (Han, Yi)[ 1 ] ; Guo, DS (Guo, Daosen)[ 1 ] ; Guo, QQ (Guo, Qunqun)[ 1 ] ; Li, RG (Li, Ronggui)[ 1 ]
À´Ô´³ö°æÎï: MARINE DRUGS ¾í: 10 ÆÚ: 12 Ò³: 2729-2740 DOI: 10.3390/md10122729 ³ö°æÄê: DEC 2012
±»ÒýƵ´Î: 0 (À´×Ô Web of Science)
ÒýÓõIJο¼ÎÄÏ×: 26 [ ²é¿´ Related Records ] ÒýÖ¤¹ØÏµÍ¼
ÕªÒª: Pine wilt disease (PWD), a destructive disease for pine trees, is caused by the pine wood nematode (PWN), Bursaphelenchus xylophilus and additional bacteria. In this study, extracts of Zostera marina showed a high nematicidal activity against PWN and some of the bacteria that it carries. Light yellow crystals were obtained from extracts of Z. marina through solvent extraction, followed by chromatography on AB-8 resin and crystallization. The NMR and HPLC analysis showed that the isolated compound was rosmarinic acid (RosA). RosA showed effective nematicidal activity, of which the LC50 (50% lethal concentration) to PWN at 24 h, 48 h and 72 h was 1.18 mg/g, 1.05 mg/g and 0.95 mg/g, respectively. To get a high yield rate of RosA from Z. marina, single factor experiments and an L-9 (3(4)) orthogonal experiment were performed. This extraction process involved 70% ethanol for 3 h at 40 degrees C. The extraction dosage was 1:50 (w/v). The highest yield of RosA from Zostera was 3.13 mg/g DW (dried weight). The crude extracts of Zostera marina (10 mg/mL) and RosA (1 mg/mL) also showed inhibitory effects to some bacterial strains carried by PWN: Klebsiella sp., Stenotrophomonas maltophilia, Streptomyces sp. and Pantoea agglomerans. The results of these studies provide clues for preparing pesticide to control PWD from Z. marina.
Èë²ØºÅ: WOS:000312607700009
ÎÄÏ×ÀàÐÍ: Article
ÓïÖÖ: English
×÷Õ߹ؼü´Ê: Zostera marina; Bursaphelenchus xylophilus; rosmarinic acid; nematicidal activity; anti-bacterial activity
KeyWords Plus: PLANT ESSENTIAL OILS; BURSAPHELENCHUS-XYLOPHILUS; ZOSTERA-MARINA; IDENTIFICATION; DERIVATIVES; COMPONENTS
ͨѶ×÷ÕßµØÖ·: Li, RG (ͨѶ×÷Õß),Qingdao Univ, Dept Biol, Qingdao 266071, Peoples R China.
µØÖ·:
[ 1 ] Qingdao Univ, Dept Biol, Qingdao 266071, Peoples R China
µç×ÓÓʼþµØÖ·: jingyu501@163.com; panxueru@sohu.com; karissahy@163.com; guodaosen@sohu.com; gqunqun@163.com; lrg@qdu.edu.cn
»ù½ð×ÊÖúÖÂл:
»ù½ð×ÊÖú»ú¹¹ ÊÚȨºÅ
Natural Science Foundation of Shandong Province, China ZR2010CM009
Basic Research Project of Qingdao, China 12-1-4-2-(1)-jch
National Natural Science Foundation of China 31070575
[ÏÔʾ»ù½ð×ÊÖúÐÅÏ¢][Òþ²Ø»ù½ð×ÊÖúÐÅÏ¢]
This work was supported in part by the Natural Science Foundation of Shandong Province (No. ZR2010CM009), China, the Basic Research Project of Qingdao (No. 12-1-4-2-(1)-jch), China and the National Natural Science Foundation of China (No. 31070575).
³ö°æÉÌ: MDPI AG, POSTFACH, CH-4005 BASEL, SWITZERLAND
Web of Science Àà±ð: Chemistry, Medicinal
Ñо¿·½Ïò: Pharmacology & Pharmacy
IDS ºÅ: 058AY
ISSN: 1660-3397 |
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