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【答案】应助回帖
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感谢参与，应助指数 +1
jingyu501: 金币+20, ★★★★★最佳答案, thanks 2013-03-09 16:57:38
Rosmarinic Acid from Eelgrass Shows Nematicidal and Antibacterial Activities against Pine Wood Nematode and Its Carrying Bacteria
作者: Wang, JY (Wang, Jingyu)[ 1 ] ; Pan, XR (Pan, Xueru)[ 1 ] ; Han, Y (Han, Yi)[ 1 ] ; Guo, DS (Guo, Daosen)[ 1 ] ; Guo, QQ (Guo, Qunqun)[ 1 ] ; Li, RG (Li, Ronggui)[ 1 ]
来源出版物: MARINE DRUGS 卷: 10 期: 12 页: 2729-2740 DOI: 10.3390/md10122729 出版年: DEC 2012
被引频次: 0 (来自 Web of Science)
引用的参考文献: 26 [ 查看 Related Records ] 引证关系图
摘要: Pine wilt disease (PWD), a destructive disease for pine trees, is caused by the pine wood nematode (PWN), Bursaphelenchus xylophilus and additional bacteria. In this study, extracts of Zostera marina showed a high nematicidal activity against PWN and some of the bacteria that it carries. Light yellow crystals were obtained from extracts of Z. marina through solvent extraction, followed by chromatography on AB-8 resin and crystallization. The NMR and HPLC analysis showed that the isolated compound was rosmarinic acid (RosA). RosA showed effective nematicidal activity, of which the LC50 (50% lethal concentration) to PWN at 24 h, 48 h and 72 h was 1.18 mg/g, 1.05 mg/g and 0.95 mg/g, respectively. To get a high yield rate of RosA from Z. marina, single factor experiments and an L-9 (3(4)) orthogonal experiment were performed. This extraction process involved 70% ethanol for 3 h at 40 degrees C. The extraction dosage was 1:50 (w/v). The highest yield of RosA from Zostera was 3.13 mg/g DW (dried weight). The crude extracts of Zostera marina (10 mg/mL) and RosA (1 mg/mL) also showed inhibitory effects to some bacterial strains carried by PWN: Klebsiella sp., Stenotrophomonas maltophilia, Streptomyces sp. and Pantoea agglomerans. The results of these studies provide clues for preparing pesticide to control PWD from Z. marina.
入藏号: WOS:000312607700009
文献类型: Article
语种: English
作者关键词: Zostera marina; Bursaphelenchus xylophilus; rosmarinic acid; nematicidal activity; anti-bacterial activity
KeyWords Plus: PLANT ESSENTIAL OILS; BURSAPHELENCHUS-XYLOPHILUS; ZOSTERA-MARINA; IDENTIFICATION; DERIVATIVES; COMPONENTS
通讯作者地址: Li, RG (通讯作者),Qingdao Univ, Dept Biol, Qingdao 266071, Peoples R China.
地址:
[ 1 ] Qingdao Univ, Dept Biol, Qingdao 266071, Peoples R China
电子邮件地址: jingyu501@163.com; panxueru@sohu.com; karissahy@163.com; guodaosen@sohu.com; gqunqun@163.com; lrg@qdu.edu.cn
基金资助致谢:
基金资助机构 授权号
Natural Science Foundation of Shandong Province, China ZR2010CM009
Basic Research Project of Qingdao, China 12-1-4-2-(1)-jch
National Natural Science Foundation of China 31070575
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This work was supported in part by the Natural Science Foundation of Shandong Province (No. ZR2010CM009), China, the Basic Research Project of Qingdao (No. 12-1-4-2-(1)-jch), China and the National Natural Science Foundation of China (No. 31070575).
出版商: MDPI AG, POSTFACH, CH-4005 BASEL, SWITZERLAND
Web of Science 类别: Chemistry, Medicinal
研究方向: Pharmacology & Pharmacy
IDS 号: 058AY
ISSN: 1660-3397 |
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