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1楼 2013-03-09 16:49:52
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hehexixi

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jingyu501: 金币+20, ★★★★★最佳答案, thanks 2013-03-09 16:57:38
Rosmarinic Acid from Eelgrass Shows Nematicidal and Antibacterial Activities against Pine Wood Nematode and Its Carrying Bacteria  
作者: Wang, JY (Wang, Jingyu)[ 1 ] ; Pan, XR (Pan, Xueru)[ 1 ] ; Han, Y (Han, Yi)[ 1 ] ; Guo, DS (Guo, Daosen)[ 1 ] ; Guo, QQ (Guo, Qunqun)[ 1 ] ; Li, RG (Li, Ronggui)[ 1 ]  
来源出版物: MARINE DRUGS  卷: 10   期: 12   页: 2729-2740   DOI: 10.3390/md10122729   出版年: DEC 2012  
被引频次: 0 (来自 Web of Science)  
引用的参考文献: 26      [ 查看 Related Records ]     引证关系图      
摘要: Pine wilt disease (PWD), a destructive disease for pine trees, is caused by the pine wood nematode (PWN), Bursaphelenchus xylophilus and additional bacteria. In this study, extracts of Zostera marina showed a high nematicidal activity against PWN and some of the bacteria that it carries. Light yellow crystals were obtained from extracts of Z. marina through solvent extraction, followed by chromatography on AB-8 resin and crystallization. The NMR and HPLC analysis showed that the isolated compound was rosmarinic acid (RosA). RosA showed effective nematicidal activity, of which the LC50 (50% lethal concentration) to PWN at 24 h, 48 h and 72 h was 1.18 mg/g, 1.05 mg/g and 0.95 mg/g, respectively. To get a high yield rate of RosA from Z. marina, single factor experiments and an L-9 (3(4)) orthogonal experiment were performed. This extraction process involved 70% ethanol for 3 h at 40 degrees C. The extraction dosage was 1:50 (w/v). The highest yield of RosA from Zostera was 3.13 mg/g DW (dried weight). The crude extracts of Zostera marina (10 mg/mL) and RosA (1 mg/mL) also showed inhibitory effects to some bacterial strains carried by PWN: Klebsiella sp., Stenotrophomonas maltophilia, Streptomyces sp. and Pantoea agglomerans. The results of these studies provide clues for preparing pesticide to control PWD from Z. marina.  
入藏号: WOS:000312607700009  
文献类型: Article  
语种: English  
作者关键词: Zostera marina; Bursaphelenchus xylophilus; rosmarinic acid; nematicidal activity; anti-bacterial activity  
KeyWords Plus: PLANT ESSENTIAL OILS; BURSAPHELENCHUS-XYLOPHILUS; ZOSTERA-MARINA; IDENTIFICATION; DERIVATIVES; COMPONENTS  
通讯作者地址: Li, RG (通讯作者),Qingdao Univ, Dept Biol, Qingdao 266071, Peoples R China.  
地址:
[ 1 ] Qingdao Univ, Dept Biol, Qingdao 266071, Peoples R China  
电子邮件地址: jingyu501@163.com; panxueru@sohu.com; karissahy@163.com; guodaosen@sohu.com; gqunqun@163.com; lrg@qdu.edu.cn  
基金资助致谢:
基金资助机构 授权号
Natural Science Foundation of Shandong Province, China  ZR2010CM009  
Basic Research Project of Qingdao, China  12-1-4-2-(1)-jch  
National Natural Science Foundation of China  31070575  

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This work was supported in part by the Natural Science Foundation of Shandong Province (No. ZR2010CM009), China, the Basic Research Project of Qingdao (No. 12-1-4-2-(1)-jch), China and the National Natural Science Foundation of China (No. 31070575).

出版商: MDPI AG, POSTFACH, CH-4005 BASEL, SWITZERLAND  
Web of Science 类别: Chemistry, Medicinal  
研究方向: Pharmacology & Pharmacy  
IDS 号: 058AY  
ISSN: 1660-3397
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2楼2013-03-09 16:55:28
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3楼2013-03-09 16:59:15
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