24小时热门版块排行榜    

查看: 487  |  回复: 2
本帖产生 1 个 翻译EPI ,点击这里进行查看
当前只显示满足指定条件的回帖,点击这里查看本话题的所有回帖

canfeng2008

银虫 (正式写手)

[求助] 急求一小段生物方面的摘要中文译为英语,谢谢

叉头转录因子O亚家族1(forkhead transcription factor group O1,FoxO1)是前体脂肪细胞分化过程的重要调控因子,通过多条通路影响细胞增殖、分化、凋亡但其作用机制及信号通路在鱼类的研究中尚不清楚。本项目拟以我国重要经济鱼类一草鱼为实验材料,采用细胞培养、RT-PCR、实时定量PCR、载体构建、免疫组化、细胞转染、Western blotting及免疫共沉淀结合质谱鉴定技术等技术手段,以草鱼脂肪细胞为体外模型,以FoxO1调控转录因子PPARγ的活性、RES和Wortmannin阻断剂对FoxO1的影响及磷酸化FoxO1的亚细胞定位和作用为切入点,研究FoxO1基因对草鱼脂肪细胞增殖分化的调控作用及信号通路。研究结果不仅能为阐明脂质沉积的复杂网络调节机制奠定基础,而且还可改为善鱼肉质量与风味提供依据,具有重强的理论及应用意义。

[ Last edited by canfeng2008 on 2013-3-7 at 08:10 ]

» 猜你喜欢

已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

canfeng2008

银虫 (正式写手)

翻的不是很好,还有人帮助吗
3楼2013-03-08 08:09:10
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
查看全部 3 个回答

Uniaxial

禁虫 (著名写手)

【答案】应助回帖

★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★
phu_grassman: 金币+5, thanks for your kind help. 2013-03-14 19:45:37
canfeng2008: 金币+20, 翻译EPI+1, 有帮助, 翻的真的不好,不过也辛苦了,谢谢 2013-03-19 22:12:03
叉头转录因子O亚家族1(forkhead transcription factor group O1,FoxO1)是前体脂肪细胞分化过程的重要调控因子,通过多条通路影响细胞增殖、分化、凋亡但其作用机制及信号通路在鱼类的研究中尚不清楚。
翻译:FoxO1 is an important regulator in preadipocytes differentiation ,and it infulences the proliferation ,differentiation and apoptosis of cells through multiple pathways while the mechanism and signal pathways in the research of fish is not clear .

本项目拟以我国重要经济鱼类一草鱼为实验材料,采用细胞培养、RT-PCR、实时定量PCR、载体构建、免疫组化、细胞转染、Western blotting及免疫共沉淀结合质谱鉴定技术等技术手段,以草鱼脂肪细胞为体外模型,以FoxO1调控转录因子PPARγ的活性、RES和Wortmannin阻断剂对FoxO1的影响及磷酸化FoxO1的亚细胞定位和作用为切入点,研FoxO1基因对草鱼脂肪细胞增殖分化的调控作用及信号通路。
翻译:This project intends to choose grass carp,one of our impotant commercial fishes,as experimental materials.Cell culture , RT-PCR , real-time quantitative  PCR , vector construction, immunohistochemistry , cell transfection , Western blotting and co-immunoprecipitation combined with mass spectrometric identification technology as the means.Adipocytes  grass carp as the vitro model. Great priorities of the research were given to the control of the activity of transcription actor PPARγ by FoxO1,the influence of RES and Wortmannin blockers on FoxO1 and  the locallization and function of phosphorylated FoxO1 subcell ,in order to investigate on the modulation and signalpathways of the proliferation and differentiation of grass carp cell by FoxO1.

研究结果不仅能为阐明脂质沉积的复杂网络调节机制奠定基础,而且还可改为善鱼肉质量与风味提供依据,具有重强的理论及应用意义。
翻译:The research result will not only lay the foundation for the clarification of the regulatory mechanism of the complex network of lipid storage,but also provide the basis for improving the flavor and quality of fish,and it is of great significantce theoretically and practically
2楼2013-03-07 15:56:01
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
最具人气热帖推荐 [查看全部] 作者 回/看 最后发表
[考研] 0854 考研调剂 招生了!AI 方向 +5 pk3725069 2026-03-19 17/850 2026-03-24 17:30 by zhouxuan..
[考研] 【考研调剂】化学专业 281分,一志愿四川大学,诚心求调剂 +14 吃吃吃才有意义 2026-03-19 15/750 2026-03-24 17:28 by dick_runner
[考研] 一志愿211 初试270分 求调剂 +5 谷雨上岸 2026-03-23 6/300 2026-03-24 16:32 by laoshidan
[考研] 招08考数学 +6 laoshidan 2026-03-20 15/750 2026-03-24 16:22 by laoshidan
[考研] 求调剂一志愿武汉理工大学材料工程(085601) +5 WW.' 2026-03-23 7/350 2026-03-24 14:50 by sprinining
[考研] 【双一流院校新能源、环境材料,材料加工与模拟招收大量调剂】 +4 Higraduate 2026-03-22 7/350 2026-03-24 11:23 by 种大树
[考研] 求材料,环境专业调剂 +3 18567500178 2026-03-18 3/150 2026-03-23 23:50 by 热情沙漠
[考研] 化学308分求调剂 +3 你好明天你好 2026-03-23 3/150 2026-03-23 20:11 by macy2011
[考研] 一志愿武理材料工程348求调剂 +6  ̄^ ̄゜汗 2026-03-19 9/450 2026-03-23 19:53 by pswait
[考研] 352求调剂 +3 大米饭! 2026-03-22 3/150 2026-03-22 23:28 by king123!
[考研] 319求调剂 +4 小力气珂珂 2026-03-20 4/200 2026-03-22 15:53 by ColorlessPI
[考研] 311求调剂 +3 26研0 2026-03-20 3/150 2026-03-22 14:46 by ColorlessPI
[考研] 生物学调剂 +5 Surekei 2026-03-21 5/250 2026-03-22 14:39 by tcx007
[考研] 318求调剂 +4 plum李子 2026-03-21 7/350 2026-03-22 14:17 by ColorlessPI
[考研] 311求调剂 +3 勇敢的小吴 2026-03-20 3/150 2026-03-21 17:40 by ColorlessPI
[考研] 307求调剂 +3 余意卿 2026-03-18 3/150 2026-03-21 17:31 by ColorlessPI
[考研] 308求调剂 +3 阿姐阿姐家啊 2026-03-18 3/150 2026-03-20 23:24 by JourneyLucky
[考研] 材料与化工 322求调剂 +4 然11 2026-03-19 4/200 2026-03-20 22:12 by luoyongfeng
[考研] 一志愿南理工085701环境302求调剂院校 +3 葵梓卫队 2026-03-20 3/150 2026-03-20 19:28 by zhukairuo
[考研] 085410人工智能专硕317求调剂(0854都可以) +4 xbxudjdn 2026-03-18 4/200 2026-03-20 09:07 by 不168
信息提示
请填处理意见