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Uniaxial
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- ×¢²á: 2012-04-20
- ÐÔ±ð: GG
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phu_grassman: ½ð±Ò+5, thanks for your kind help. 2013-03-14 19:45:37
canfeng2008: ½ð±Ò+20, ·ÒëEPI+1, ¡ïÓаïÖú, ·µÄÕæµÄ²»ºÃ£¬²»¹ýÒ²ÐÁ¿àÁË£¬Ð»Ð» 2013-03-19 22:12:03
phu_grassman: ½ð±Ò+5, thanks for your kind help. 2013-03-14 19:45:37
canfeng2008: ½ð±Ò+20, ·ÒëEPI+1, ¡ïÓаïÖú, ·µÄÕæµÄ²»ºÃ£¬²»¹ýÒ²ÐÁ¿àÁË£¬Ð»Ð» 2013-03-19 22:12:03
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²æÍ·×ªÂ¼Òò×ÓOÑǼÒ×å1£¨forkhead transcription factor group O1£¬FoxO1£©ÊÇǰÌåÖ¬·¾Ï¸°û·Ö»¯¹ý³ÌµÄÖØÒªµ÷¿ØÒò×Ó£¬Í¨¹ý¶àÌõͨ·ӰÏìϸ°ûÔöÖ³¡¢·Ö»¯¡¢µòÍöµ«Æä×÷ÓûúÖÆ¼°ÐźÅͨ·ÔÚÓãÀàµÄÑо¿ÖÐÉв»Çå³þ¡£ ·Ò룺FoxO1 is an important regulator in preadipocytes differentiation ,and it infulences the proliferation ,differentiation and apoptosis of cells through multiple pathways while the mechanism and signal pathways in the research of fish is not clear . ±¾ÏîÄ¿ÄâÒÔÎÒ¹úÖØÒª¾¼ÃÓãÀàÒ»²ÝÓãΪʵÑé²ÄÁÏ£¬²ÉÓÃϸ°ûÅàÑø¡¢RT-PCR¡¢ÊµÊ±¶¨Á¿PCR¡¢ÔØÌå¹¹½¨¡¢ÃâÒß×黯¡¢Ï¸°ûתȾ¡¢Western blotting¼°ÃâÒß¹²³Áµí½áºÏÖÊÆ×¼ø¶¨¼¼ÊõµÈ¼¼ÊõÊֶΣ¬ÒÔ²ÝÓãÖ¬·¾Ï¸°ûΪÌåÍâÄ£ÐÍ£¬ÒÔFoxO1µ÷¿Ø×ªÂ¼Òò×ÓPPAR¦ÃµÄ»îÐÔ¡¢RESºÍWortmannin×è¶Ï¼Á¶ÔFoxO1µÄÓ°Ïì¼°Á×ËữFoxO1µÄÑÇϸ°û¶¨Î»ºÍ×÷ÓÃΪÇÐÈëµã£¬ÑÐFoxO1»ùÒò¶Ô²ÝÓãÖ¬·¾Ï¸°ûÔöÖ³·Ö»¯µÄµ÷¿Ø×÷Óü°ÐźÅͨ·¡£ ·Ò룺This project intends to choose grass carp£¬one of our impotant commercial fishes£¬as experimental materials.Cell culture , RT-PCR , real-time quantitative PCR , vector construction, immunohistochemistry , cell transfection , Western blotting and co-immunoprecipitation combined with mass spectrometric identification technology as the means.Adipocytes grass carp as the vitro model. Great priorities of the research were given to the control of the activity of transcription actor PPAR¦Ã by FoxO1£¬the influence of RES and Wortmannin blockers on FoxO1 and the locallization and function of phosphorylated FoxO1 subcell £¬in order to investigate on the modulation and signalpathways of the proliferation and differentiation of grass carp cell by FoxO1. Ñо¿½á¹û²»½öÄÜΪ²ûÃ÷Ö¬ÖʳÁ»ýµÄ¸´ÔÓÍøÂçµ÷½Ú»úÖÆµì¶¨»ù´¡£¬¶øÇÒ»¹¿É¸ÄÎªÉÆÓãÈâÖÊÁ¿Óë·çζÌṩÒÀ¾Ý£¬¾ßÓÐÖØÇ¿µÄÀíÂÛ¼°Ó¦ÓÃÒâÒå¡£ ·Ò룺The research result will not only lay the foundation for the clarification of the regulatory mechanism of the complex network of lipid storage£¬but also provide the basis for improving the flavor and quality of fish£¬and it is of great significantce theoretically and practically |
2Â¥2013-03-07 15:56:01
canfeng2008
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- ×¢²á: 2008-01-09
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